Geadas Farias Pedro, Gama Fernando, Reis Diogo, Alarico Susana, Empadinhas Nuno, Martins José Carlos, de Almeida Ana Figueiredo, Morais Paula Vasconcelos
Instituto Piaget, Silves, Portugal.
CEMUC-Department of Mechanical Engineering, University of Coimbra, Coimbra, Portugal.
Antonie Van Leeuwenhoek. 2017 Jul;110(7):863-876. doi: 10.1007/s10482-017-0857-z. Epub 2017 Mar 23.
Hospital environmental conditions, human occupancy, and the characteristics of the equipment influence the survival of microbial communities and raise a concern with regard to nosocomial infections. The objective of the present work was to use the monitoring of Pseudomonas aeruginosa, Klebsiella spp. and non-tuberculous mycobacteria as a strategy to improve knowledge on microbial colonization of non-critical equipment and surfaces, in a tertiary hospital from Central Portugal. A 3-month microbiological survey was performed in a district teaching hospital. A total of 173 samples were obtained from the wards Hematology, Urology, Medicine, and Renal Transplants, and 102 presumptive strains recovered. Per sampling, Pseudomonas Isolation agar showed 42.8 to 73.3% of presumptive P. aeruginosa colonies and MacConkey agar recovered mostly Staphylococcus. Most of the colonies recovered in Middlebrook 7H10-PANTA belonged to the genus Methylobacterium. Taps and WC shower curtains carry high bacterial species diversity. The Redundancy Analysis grouped the samples in those mostly handled by patients, and those mostly handled by healthcare staff or of mixed use. This study shows that the preferential users of the space and equipment seem to be important contributors to the microbial community. The most recovered genus was Methylobacterium, known as colonizer of the water distribution system therefore, it is possible that the water points and biofilms in taps also contribute as dispersion hotspots.
医院的环境条件、人员占用情况以及设备特性会影响微生物群落的存活，并引发对医院感染的担忧。本研究的目的是通过监测铜绿假单胞菌、克雷伯菌属和非结核分枝杆菌，来提高对葡萄牙中部一家三级医院非关键设备和表面微生物定植情况的了解。在一家地区教学医院进行了为期3个月的微生物学调查。从血液科、泌尿科、内科和肾移植病房共采集了173份样本，分离出102株疑似菌株。每次采样时，假单胞菌分离琼脂显示42.8%至73.3%的疑似铜绿假单胞菌菌落，而麦康凯琼脂主要分离出葡萄球菌。在Middlebrook 7H10 - PANTA培养基上分离出的大多数菌落属于甲基杆菌属。水龙头和卫生间浴帘携带的细菌种类多样性很高。冗余分析将样本分为主要由患者接触的样本、主要由医护人员接触的样本或混合使用的样本。这项研究表明，空间和设备的主要使用者似乎是微生物群落的重要贡献者。分离出最多的属是甲基杆菌属，该属是水分配系统的定植菌，因此，水龙头中的水龙头和生物膜也可能是传播热点。
