Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul, South Korea.
Department of Molecular Science and Technology, Ajou University, Suwon, South Korea.
Sci Rep. 2017 Mar 24;7:44847. doi: 10.1038/srep44847.
MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate gene expression by suppressing translation or facilitating mRNA decay. Differential expression of miRNAs is involved in the pathogenesis of several diseases including cancer. Here, we investigated the role of-miR-24-3p as a downregulated miRNA in metastatic cancer. miR-24-3p was decreased in metastatic cancer and lower expression of miR-24-3p was related to poor survival of cancer patients. Consistently, ectopic expression of miR-24-3p suppressed the cell migration, invasion, and proliferation of MCF7, Hep3B, B16F10, SK-Hep1, and PC-3 cells by directly targeting p130Cas. Stable expression of p130Cas restored miR-24-3p-mediated inhibition of cell migration and invasion. These results suggest that miR-24-3p functions as a tumor suppressor and the miR-24-3p/p130Cas axis is a novel factor of cancer progression by regulating cell migration and invasion.
微小 RNA（miRNAs）是一类小的非编码 RNA，通过抑制翻译或促进 mRNA 降解来负调控基因表达。miRNAs 的差异表达参与了多种疾病的发病机制，包括癌症。在这里，我们研究了-miR-24-3p 作为转移性癌症中下调 miRNA 的作用。miR-24-3p 在转移性癌症中减少，miR-24-3p 的低表达与癌症患者的不良预后相关。一致地，miR-24-3p 的异位表达通过直接靶向 p130Cas 抑制 MCF7、Hep3B、B16F10、SK-Hep1 和 PC-3 细胞的迁移、侵袭和增殖。p130Cas 的稳定表达恢复了 miR-24-3p 介导的细胞迁移和侵袭抑制。这些结果表明，miR-24-3p 作为肿瘤抑制因子发挥作用，miR-24-3p/p130Cas 轴通过调节细胞迁移和侵袭是癌症进展的一个新的因素。
