Mokhtari Mohammad Javad, Koohpeima Fatemeh, Mohammadi Hadi
Young Researchers and Elite Club, Zarghan Branch, Islamic Azad University, Zarghan, Iran.
Department of Operative Dentistry, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
Chem Biol Drug Des. 2017 Oct;90(4):618-628. doi: 10.1111/cbdd.12985. Epub 2017 May 14.
To date, high mortality in women due to malignancy breast cancer related to the metastasis to the bone is a significant challenge. As, magnetic nanoparticles (MNPs) conjugated with the biocompatible polymers was employed for the delivery of some hydrophobic anticancer agents, the main aim of the current research was to assess whether cisplatin-loaded MNPs enhanced the anticancer effect of free cisplatin in breast cancer cells. MNPs decorated with PEG were synthesized by an improved coprecipitation technique, and then cisplatin was loaded onto the MNPs via a simple mixing method. Afterward, its morphology, size, chemical structure, magnetic property, hydrodynamic diameter, zeta potential, and crystal structure were characterized by scanning and transmittance electron microscopy, Fourier transforms infrared spectroscopy, vibrating sample magnetometer, dynamic light scattering, and X-ray powder diffraction and flame atomic absorption spectroscopy respectively. Additionally, the effects of cisplatin and MNPs-PEG-cisplatin on viability, migration and adhesion capacity of T47D cells were investigated by evaluating α2-integrin and β1-integrin; mRNAs were assessed by real-time RT-PCR. Consequently, the in vitro assay results showed a considerable dose-dependent inhibitory effect of cisplatin and MNPs-PEG-cisplatin on proliferation, migration, and adhesion of T47D cells. Finally, current research was shown that MNPs-PEG-cisplatin strongly increased anticancer effects compared with free cisplatin in the T47D cell line.
迄今为止，女性因恶性乳腺癌骨转移导致的高死亡率是一项重大挑战。由于与生物相容性聚合物共轭的磁性纳米颗粒（MNPs）被用于递送一些疏水性抗癌药物，当前研究的主要目的是评估顺铂负载的MNPs是否能增强游离顺铂对乳腺癌细胞的抗癌效果。通过改进的共沉淀技术合成了聚乙二醇修饰的MNPs，然后通过简单的混合方法将顺铂负载到MNPs上。之后，分别通过扫描电子显微镜、透射电子显微镜、傅里叶变换红外光谱、振动样品磁强计、动态光散射、X射线粉末衍射和火焰原子吸收光谱对其形态、尺寸、化学结构、磁性、流体动力学直径、zeta电位和晶体结构进行了表征。此外，通过评估α2整合素和β1整合素，研究了顺铂和MNPs-PEG-顺铂对T47D细胞活力、迁移和黏附能力的影响；通过实时RT-PCR评估mRNA。因此，体外试验结果显示顺铂和MNPs-PEG-顺铂对T47D细胞的增殖、迁移和黏附具有显著的剂量依赖性抑制作用。最后，当前研究表明，与游离顺铂相比，MNPs-PEG-顺铂在T47D细胞系中能显著增强抗癌效果。
