WRKY转录因子PtrWRKY18和PtrWRKY35促进杨树对落叶松-杨栅锈菌的抗性。

The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus.

作者信息

Jiang Yuanzhong, Guo Li, Ma Xiaodong, Zhao Xin, Jiao Bo, Li Chaofeng, Luo Keming

机构信息

Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.

MOE Key Laboratory of Bio-Resources and Eco-Environment, College of Life Science, Sichuan University, No. 24, South Section 1, Yihuan Road, Chengdu 610065, China.

出版信息

Tree Physiol. 2017 May 1;37(5):665-675. doi: 10.1093/treephys/tpx008.

DOI:10.1093/treephys/tpx008

PMID:28338710

Abstract

WRKY transcription factors play important roles in response to diverse environmental stresses, but exact functions of these proteins in poplar defense are still largely unknown. In a previous study, we have shown that poplar WRKY89 is induced by salicylic acid (SA) treatment and plays an important role in resistance against fungi in transgenic poplars. Here, we determined an increase in transcript levels of Group IIa WRKY members in transgenic poplars overexpressing WRKY89 using quantitative real-time polymerase chain reaction analysis. Yeast one-hybrid assay showed that PtrWRKY18 and PtrWRKY35 were potential target genes of WRKY89. Furthermore, we demonstrated that PtrWRKY18 and PtrWRKY35 were localized in the nucleus, and exhibited no transcription activation activity. Constitutive overexpression of PtrWRKY18 and PtrWRKY35 in poplars activated pathogenesis-related genes, and increased resistance to the biotrophic pathogen Melampsora. The results also provided support for the involvement of SA-mediated signaling in Melampsora resistance.

摘要

WRKY转录因子在应对多种环境胁迫中发挥重要作用，但这些蛋白在杨树防御中的具体功能仍 largely未知。在先前的一项研究中，我们已表明杨树WRKY89受水杨酸（SA）处理诱导，并在转基因杨树对真菌的抗性中发挥重要作用。在此，我们通过定量实时聚合酶链反应分析确定，在过表达WRKY89的转基因杨树中，IIa类WRKY成员的转录水平有所增加。酵母单杂交试验表明，PtrWRKY18和PtrWRKY35是WRKY89的潜在靶基因。此外，我们证明PtrWRKY18和PtrWRKY35定位于细胞核，且不表现出转录激活活性。PtrWRKY18和PtrWRKY35在杨树中的组成型过表达激活了病程相关基因，并增强了对活体营养型病原菌梅氏锈菌的抗性。这些结果也为SA介导的信号传导参与梅氏锈菌抗性提供了支持。