超氧化物和脂质过氧化物对培养的猪主动脉内皮细胞胞质游离钙浓度的影响。

Effect of superoxide and lipid peroxide on cytosolic free calcium concentration in cultured pig aortic endothelial cells.

作者信息

Hirosumi J, Ouchi Y, Watanabe M, Kusunoki J, Nakamura T, Orimo H

机构信息

Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan.

出版信息

Biochem Biophys Res Commun. 1988 Apr 15;152(1):301-7. doi: 10.1016/s0006-291x(88)80714-9.

DOI:10.1016/s0006-291x(88)80714-9

PMID:2833890

Abstract

The effect of reactive oxygen on cytosolic free calcium concentration [( Ca++]i) in pig aortic endothelial cells (ECs) was studied. Linoleate hydroperoxide (LHO) and superoxide radicals generated from xanthine with xanthine oxidase (X-XO) were used as sources of reactive oxygen. [Ca++]i in ECs was measured with quin 2 and the value for quiescent ECs was 112 +/- 11 nM. Both LHO and X-XO increased [Ca++]i in a dose-dependent manner without accompanying the significant cellular damage. Nifedipine suppressed the increase in [Ca++]i provoked by LHO and X-XO. Thus, the biological effects of reactive oxygen might be mediated, at least in part, by the activation of voltage-dependent calcium channels in ECs.

摘要

研究了活性氧对猪主动脉内皮细胞（ECs）胞质游离钙浓度[（Ca++]i）的影响。以亚油酸氢过氧化物（LHO）和黄嘌呤与黄嘌呤氧化酶（X-XO）产生的超氧自由基作为活性氧来源。用喹啉2测定ECs中的[Ca++]i，静息ECs的值为112±11 nM。LHO和X-XO均以剂量依赖性方式增加[Ca++]i，且不伴有明显的细胞损伤。硝苯地平抑制LHO和X-XO引起的[Ca++]i升高。因此，活性氧的生物学效应可能至少部分是由ECs中电压依赖性钙通道的激活介导的。