在溴化乙锭存在的情况下，用脾磷酸二酯酶对酵母苯丙氨酸tRNA进行可控降解。
Controlled degradation of yeast tRNAPhe by spleen phosphodiesterase in the presence of ethidium bromide.
作者信息
Lesnikowski Z J
机构信息
Polish Academy of Sciences, Department of Bioorganic Chemistry, Boczna.
出版信息
Biochem Biophys Res Commun. 1988 Apr 15;152(1):477-83. doi: 10.1016/s0006-291x(88)80738-1.
PMID:2833899
Abstract
Degradation of yeast tRNAPhe with spleen phosphodiesterase in the presence of ethidium bromide has been studied. It was found that in the presence of the intercalating dye, the digestion is halted after a limited number of nucleotides is removed. Possible explanations of the observed phenomenon in connection with tRNA-ethidium bromide complex formation are discussed.
摘要
研究了在溴化乙锭存在的情况下，用脾磷酸二酯酶降解酵母苯丙氨酸转运核糖核酸（tRNAPhe）的情况。发现在嵌入染料存在的情况下，去除有限数量的核苷酸后消化就会停止。讨论了与tRNA - 溴化乙锭复合物形成相关的观察到的现象的可能解释。
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