来自嗜碱芽孢杆菌RAB的两种可溶性细胞色素的纯化与特性分析
Purification and characterization of two soluble cytochromes from the alkalophile Bacillus firmus RAB.
作者信息
Davidson M W, Gray K A, Knaff D B, Krulwich T A
机构信息
Department of Biochemistry, Mount Sinai School of Medicine, City University of New York, NY 10029.
出版信息
Biochim Biophys Acta. 1988 May 11;933(3):470-7. doi: 10.1016/0005-2728(88)90082-5.
A soluble cytochrome c and soluble cytochrome b were purified from the alkalophilic Bacillus firmus RAB. The cytochrome c, with an alpha band at 552 nm, had an apparent molecular weight of 16,500 and was acidic, with a pI of 3.4. At both pH 7.0 and 8.3, the midpoint potential of c-552 was +66 mV. Above pH 8.3, the cytochrome exhibited a pH-dependent decrease in midpoint potential. This property, among others, distinguished the cytochrome c-552 from other membrane-associated c-type cytochromes. The soluble cytochrome b, with an alpha band maximum at 558 nm, had a molecular weight of approx. 15,500 and was also an acidic protein, with a pI of 3.07. It exhibited a pH-independent midpoint potential of +28 mV.
从嗜碱芽孢杆菌RAB中纯化出了一种可溶性细胞色素c和一种可溶性细胞色素b。细胞色素c在552 nm处有一条α带，表观分子量为16,500，呈酸性，pI为3.4。在pH 7.0和8.3时，c-552的中点电位均为+66 mV。在pH 8.3以上，该细胞色素的中点电位呈现出pH依赖性下降。这一特性以及其他特性使细胞色素c-552有别于其他与膜相关的c型细胞色素。可溶性细胞色素b在558 nm处有最大α带，分子量约为15,500，也是一种酸性蛋白，pI为3.07。它表现出+28 mV的与pH无关的中点电位。
Zotero 插件