专性甲基营养菌嗜甲基甲基ophilus甲基营养菌可溶性细胞色素c的纯化及性质
The purification and properties of the soluble cytochromes c of the obligate methylotroph Methylophilus methylotrophus.
作者信息
Cross A R, Anthony C
出版信息
Biochem J. 1980 Nov 15;192(2):421-7. doi: 10.1042/bj1920421.
The obligate methylotroph Methylophilus methylotrophus contains three distinct soluble cytochromes c. The major cytochromes, cytochrome cH (about 50% of the total) and cytochrome cL (about 42%), were similar in most respects to the cytochromes cH and cL of the facultative methylotroph Pseudomonas AM1 [O'Keeffe & Anthony (1980) Biochem. J. 192, 411-419]. Cytochrome cH had a high isoelectric point, a midpoint redox potential at pH 7.0 of 373 mV and a low molecular weight (8500). The cytochrome cL had a low isoelectric point, a midpoint potential of 310 mV and a molecular weight of 21,000. The third cytochrome, cytochrome cLM, was clearly distinct from cytochromes cH and cL. Like the cytochrome cL of Pseudomonas AM1, the cytochrome cL of M. methylotrophus had the lowest midpoint potential, it reacted most rapidly with methanol dehydrogenase and it combined to the greatest extent with CO. Cytochromes cH and L of M. methylotrophus differed from those from Pseudomonas AM1 in having unusually high midpoint redox potentials for non-photosynthetic bacteria and in exhibiting a split alpha-band at low temperatures.
专性甲基营养菌嗜甲基甲基ophilus含有三种不同的可溶性细胞色素c。主要的细胞色素，细胞色素cH（约占总量的50%）和细胞色素cL（约占42%），在大多数方面与兼性甲基营养菌假单胞菌AM1的细胞色素cH和cL相似[奥基夫和安东尼（1980年）《生物化学杂志》192卷，411 - 419页]。细胞色素cH具有高的等电点，在pH 7.0时的中点氧化还原电位为373 mV，分子量低（8500）。细胞色素cL具有低的等电点，中点电位为310 mV，分子量为21,000。第三种细胞色素，细胞色素cLM，明显不同于细胞色素cH和cL。与假单胞菌AM1的细胞色素cL一样，嗜甲基甲基ophilus的细胞色素cL具有最低的中点电位，它与甲醇脱氢酶反应最快，并且与CO结合的程度最大。嗜甲基甲基ophilus的细胞色素cH和L与假单胞菌AM1的不同之处在于，对于非光合细菌来说，它们具有异常高的中点氧化还原电位，并且在低温下呈现分裂的α带。
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