Neganova Anastasiia Y, Postnov Dmitry D, Sosnovtseva Olga, Jacobsen Jens Christian B
Department of Biomedical Sciences, University of Copenhagen, Blegdamsvej 3, 2200 Copenhagen N, Denmark.
PLoS One. 2017 Mar 24;12(3):e0173805. doi: 10.1371/journal.pone.0173805. eCollection 2017.
Vasomotion is spontaneous or induced rhythmic changes in vascular tone or vessel diameter that lead to rhythmic changes in flow. While the vascular research community debates the physiological and pathophysiological consequence of vasomotion, there is a great need for experimental techniques that can address the role and dynamical properties of vasomotion in vivo. We apply laser speckle imaging to study spontaneous and drug induced vasomotion in retinal network of anesthetized rats. The results reveal a wide variety of dynamical patterns. Wavelet-based analysis shows that (i) spontaneous vasomotion occurs in anesthetized animals and (ii) vasomotion can be initiated by systemic administration of the thromboxane analogue U-46619 and the nitric-oxide donor S-nitroso-acetylDL-penicillamine (SNAP). Although these drugs activate different cellular pathways responsible for vasomotion, our approach can track the dynamical changes they cause.
血管运动是指血管张力或血管直径的自发或诱导性节律变化，这些变化会导致血流的节律性改变。尽管血管研究界对血管运动的生理和病理生理后果存在争议，但迫切需要能够研究血管运动在体内的作用和动力学特性的实验技术。我们应用激光散斑成像技术来研究麻醉大鼠视网膜网络中的自发和药物诱导的血管运动。结果揭示了各种各样的动力学模式。基于小波的分析表明：（i）麻醉动物中会发生自发血管运动；（ii）血栓素类似物U-46619和一氧化氮供体S-亚硝基乙酰-DL-青霉胺（SNAP）的全身给药可引发血管运动。尽管这些药物激活了负责血管运动的不同细胞途径，但我们的方法可以追踪它们引起的动力学变化。
