Department of Poultry Science, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville 72701.
USDA-ARS Poultry Production and Product Safety Research, University of Arkansas, Fayetteville 72701.
Poult Sci. 2017 Jul 1;96(7):2145-2150. doi: 10.3382/ps/pex009.
An in vitro experiment was performed to determine if biofilm would develop when polyvinylchloride (PVC) test coupons (material used for poultry waterlines) were exposed to low bacterial content warm water (≤1000 cfu/mL, test water) and also to determine if biofilm development would be influenced by adding a sanitizer. PVC sections 2.54 cm long and internal diameter of 1. 90 cm were used as test coupons to grow biofilm. Two coupons were immersed in 600 mL test water in a beaker. Nine beakers were utilized similarly with a total of 18 coupons. Three beakers (T1) were treated with a chlorine (Cl) based product (targeted to produce 2 to 4 ppm residual) and the other 3 (T2) with hydrogen peroxide a (HP) based product (targeted to produce 25 to 50 ppm residual). Three untreated beakers served as controls (T3). All beakers and coupons were placed into a water bath shaker under warm and moving water conditions mimicking poultry brooding conditions. Coupons and test water were sampled for treatments for aerobic plate count (APC). Trial 1 used test water with zero cfu bacteria/mL initial APC, whereas the Trial 2 test water initial APC was 3 log10 cfu/mL. Test water samples and coupons had no bacterial growth for all treatments on sampling occasions for Trial 1. In Trial 2, T3 (control) and T2 (HP treated) had APC growth in both test water (2.5 to 3.0 log10 cfu/mL) and on coupons (2 to 2.5 log10 cfu/cm2) on sampling d with no difference (P > 0.05) between these treatments. Whereas, T1 (Cl treated) eliminated bacteria (zero cfu/mL) in test water and inhibited biofilm growth on test coupons (≤0.2 log10 cfu/cm2) during sampling d (P < 0.05). This experiment showed that biofilm can develop in minimally contaminated water even in the presence of sanitizers, yet chlorine was more effective than hydrogen peroxide in limiting this development.
进行了一项体外实验，以确定聚氯乙烯 (PVC) 测试片（用于家禽水管的材料）暴露于低细菌含量温水（≤1000 cfu/mL，测试水）时是否会形成生物膜，并确定添加消毒剂是否会影响生物膜的形成。使用 2.54 厘米长、内径为 1.90 厘米的 PVC 段作为测试片来生长生物膜。将两个测试片浸入 600 毫升测试水中的烧杯中。类似地使用了 9 个烧杯，总共 18 个测试片。三个烧杯 (T1) 用含氯 (Cl) 的产品处理（目标产生 2 至 4 ppm 的残留），另外三个 (T2) 用基于过氢 (HP) 的产品处理（目标产生 25 至 50 ppm 的残留）。三个未处理的烧杯作为对照 (T3)。所有烧杯和测试片都被放置在水浴摇床中，在温暖和流动的水中，模拟家禽育雏条件。对测试片和测试水进行取样，用于需氧平板计数 (APC) 处理。试验 1 使用初始 APC 为零 cfu 细菌/mL 的测试水，而试验 2 的初始 APC 为 3 log10 cfu/mL。在试验 1 的取样时，所有处理的测试水和测试片都没有细菌生长。在试验 2 中，T3（对照）和 T2（HP 处理）在测试水（2.5 至 3.0 log10 cfu/mL）和测试片上（2 至 2.5 log10 cfu/cm2）均有 APC 生长，这些处理之间没有差异（P > 0.05）。然而，T1（Cl 处理）在测试水中消除了细菌（零 cfu/mL），并抑制了测试片上的生物膜生长（≤0.2 log10 cfu/cm2）在采样日（P < 0.05）。该实验表明，即使在存在消毒剂的情况下，生物膜也可以在受污染较少的水中形成，但氯比过氧氢更有效地限制这种形成。
