Dietl M M, Cortés R, Palacios J M
Preclinical Research, Sandoz Ltd., Basle, Switzerland.
Brain Res. 1988 Jan 26;439(1-2):366-71. doi: 10.1016/0006-8993(88)91496-5.
GABA/benzodiazepine (BZ) receptors were localized in the pigeon brain by in vitro receptor autoradiography using [3]flunitrazepam as ligand. Highest densities of binding sites were observed in the optic tectum, in the nucleus pretectalis, the nucleus intercollicularis and the substantia gelatinosa of the spinal cord. Intermediate densities were found in the forebrain, particularly the paleostriatum primitivum, the nucleus rotundus and the cerebellum. Low densities were detected in the midbrain and brainstem. The paleostriatum primitivum and cerebellum were enriched in receptors of the BZ type I, as indicated by their high affinity for compound CL 218872. The addition of GABA resulted in an enhanced binding of the ligand in all brain regions. All these results indicate that GABA/BZ receptors in the pigeon brain present similar pharmacological properties and comparable anatomical distribution to those in mammalian brain.
利用[3]氟硝西泮作为配体，通过体外受体放射自显影术将γ-氨基丁酸/苯二氮䓬（GABA/BZ）受体定位在鸽子大脑中。在视顶盖、顶盖前核、中脑间核和脊髓胶状质中观察到结合位点的最高密度。在前脑，特别是原新纹状体、圆核和小脑中发现中等密度。在中脑和脑干中检测到低密度。原新纹状体和小脑富含I型BZ受体，这由它们对化合物CL 218872的高亲和力表明。添加GABA导致配体在所有脑区的结合增强。所有这些结果表明，鸽子大脑中的GABA/BZ受体具有与哺乳动物大脑中相似的药理学特性和可比的解剖分布。
