Domínguez Francisco, Ferrando Marcos, Díaz-Gimeno Patricia, Quintana Fernando, Fernández Gemma, Castells Inés, Simón Carlos
Fundación Instituto Valenciano de Infertilidad (FIVI), Instituto Universitario IVI (IUIVI), Paterna, Valencia, Spain.
INCLIVA Biomedical Research Institute, Valencia, Spain.
Biol Reprod. 2017 Apr 1;96(4):772-779. doi: 10.1093/biolre/iox014.
The proteomic content of the endometrial fluid (EF) from patients with endometriosis has been investigated, but the lipidomic profile has not been analyzed yet in detail.This study is a comparative untargeted lipidomic analysis of human EF obtained from 35 patients (12 endometriosis and 23 controls). Global differential lipidomic profile was analyzed in both groups by ultrahigh performance liquid chromatography coupled to mass spectrometry. A total of 123 out of the 457 metabolites identified in the EF were found to be significantly differentially expressed between ovarian endometriosis (OE) versus controls. Univariate statistical analysis showed reduced levels of saturated diacylglycerols and saturated triacylglycerols in endometriosis patients. A predictive model was generated using the 123 differentially expressed metabolites. Using this model, we were able to correctly classify 86% of the samples. This study identified the lipidomic profile in the EF associated with OE, suggesting that EF analysis could be considered as a minimally invasive approach for the diagnosis of endometriosis. In conclusion, the lipidomic profile of the EF is different between samples from patients with OE and controls.
子宫内膜异位症患者子宫内膜液（EF）的蛋白质组学内容已得到研究，但脂质组学特征尚未进行详细分析。本研究是对35例患者（12例子宫内膜异位症患者和23例对照）的人EF进行的比较非靶向脂质组学分析。通过超高效液相色谱-质谱联用对两组的整体差异脂质组学特征进行分析。在EF中鉴定出的457种代谢物中，共有123种在卵巢子宫内膜异位症（OE）与对照之间存在显著差异表达。单变量统计分析显示，子宫内膜异位症患者中饱和二酰甘油和饱和三酰甘油水平降低。利用这123种差异表达的代谢物建立了预测模型。使用该模型，我们能够正确分类86%的样本。本研究确定了与OE相关的EF中的脂质组学特征，表明EF分析可被视为诊断子宫内膜异位症的一种微创方法。总之，OE患者样本与对照样本的EF脂质组学特征不同。
