Department of Physics, Physics of Supramolecular Systems and Surfaces, University of Bielefeld , 33615 Bielefeld, Germany.
Fritz-Haber-Institut der Max-Planck-Gesellschaft, Faradayweg 4-6, 14195 Berlin, Germany.
Langmuir. 2017 Apr 18;33(15):3847-3854. doi: 10.1021/acs.langmuir.6b04207. Epub 2017 Apr 3.
We show that 4'-nitro-1,1'-biphenyl-4-thiol self-assembled monolayers (NBPT SAMs) on gold can be exchanged with 11-(mercaptoundecyl)triethylene glycol (CEGOH) SAMs via vapor deposition (VD). The pristine and the exchanged SAMs obtained by VD as well as solution method (SM) were characterized by X-ray photoelectron spectroscopy (XPS) and polarization modulation infrared reflection-absorption spectroscopy (PM-IRRAS). Using surface plasmon resonance (SPR), it is shown that CEGOH SAMs on gold obtained by vapor deposition exchange (VD) have the same protein resistivity as SAMs obtained by the direct self-assembly process. As expected, the cross-linked NBPT SAM are found to be resistive to both exchange processes, VD and solution method exchange (SM). In this way, VD opens up an elegant and new approach of patterning SAM surfaces in situ at vacuum conditions without using any solvents. By combining electron irradiation-induced chemical lithography of NBPT SAMs with VD, biofunctional patterned substrates were engineered and used for immobilization of protein arrays.
我们表明，金上的 4'-硝基-1,1'-联苯-4-硫醇自组装单层（NBPT SAM）可以通过气相沉积（VD）与 11-（巯基十一烷基）三乙二醇（CEGOH）SAM 交换。通过 X 射线光电子能谱（XPS）和偏振调制红外反射吸收光谱（PM-IRRAS）对通过 VD 以及溶液法（SM）获得的原始和交换 SAM 进行了表征。利用表面等离子体共振（SPR），表明通过气相沉积交换（VD）获得的金上的 CEGOH SAM 具有与直接自组装过程获得的 SAM 相同的蛋白质电阻率。正如预期的那样，交联的 NBPT SAM 被发现对 VD 和溶液法交换（SM）两种交换过程都具有抗性。通过这种方式，VD 在真空中开辟了一种优雅的新方法，可以原位对 SAM 表面进行图案化，而无需使用任何溶剂。通过将 NBPT SAM 的电子辐照诱导化学光刻与 VD 相结合，设计了具有生物功能的图案化基底，并用于固定蛋白质阵列。
