The trisaccharide beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)-beta-D-Manp, as its 8-methoxycarbonyloctyl glycoside, is an acceptor selective for N-acetylglucosaminyltransferase V.
作者信息
Hindsgaul O, Tahir S H, Srivastava O P, Pierce M
机构信息
Department of Chemistry, University of Alberta, Edmonton, Canada.
出版信息
Carbohydr Res. 1988 Mar 1;173(2):263-72. doi: 10.1016/s0008-6215(00)90822-x.
PMID:2834054
Abstract
Incubation of the trisaccharide acceptor, beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)-beta-D-Manp-O( CH2)8CO2Me with sonicates of Rous sarcoma-transformed baby-hamster kidney cells, which contain N-acetylglucosaminyltransferase V activity, resulted in the production of beta-D-GlcpNAc-(1----2)-[beta-D-GlcpNAc-(1----6)]-alpha-D-Manp-(1- ---6)- beta-D-Manp-O(CH2)8CO2Me (4). The product of the enzymic reaction was identified by comparison of its 1H-n.m.r. spectrum with that of authentic 4 whose chemical synthesis is also described.
摘要
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