Wing M G, Rogers K, Jacob G, Rees R C
University Surgical Unit, Northern General Hospital, Sheffield, U.K.
Cancer Immunol Immunother. 1988;26(2):169-75. doi: 10.1007/BF00205611.
Cryosurgery of a primary HSV-2-induced hamster fibrosarcoma resulted in the generation of a population of suppressor cells. These cells were detectable in the spleen 1-10 days post-cryosurgery by their ability to suppress the proliferation of immunocompetent splenic T-lymphocytes following exposure to concanavalin A (Con A). The spleens of tumour-bearing (t.b.) animals which received cryosurgery 3 days previously displayed gross splenomegaly due to the generation of large numbers of highly proliferative erythroblasts. The erythroblast cells were unlikely to be the source of suppression since time course studies have demonstrated the presence of suppressor cells before and after their appearance in the spleen. The erythroblasts therefore probably reflected a response by the host to regenerate the erythrocytes lost during surgery and their presence was independent of the appearance of suppressor cells. Characterisation of the suppressor cell has revealed it to be non-adherent and esterase negative making it unlikely to be of macrophage (MO) lineage. This was confirmed by the ability of splenic MOs from day 3 t.b. cryosurgery-treated animals to completely restore Con A-dependent T-lymphocyte proliferation following MO depletion. As nylonwool column-eluted cells are able to suppress Con A-dependent T-lymphocyte proliferation, it seemed unlikely that B-lymphocytes play a role in cryosurgery-induced immunosuppression. These findings suggest that cryosurgery of a t.b. animal results in the generation of a population of T-lymphocytes capable of suppressing Con A-dependent T-lymphocyte proliferation, and infers that these cells contribute to the inferior prognosis following cryosurgery as compared to excision of a metastatic tumour.
对原发性单纯疱疹病毒2型（HSV - 2）诱导的仓鼠纤维肉瘤进行冷冻手术，导致一群抑制细胞的产生。这些细胞在冷冻手术后1 - 10天可在脾脏中检测到，其依据是它们能够抑制免疫活性脾T淋巴细胞在接触刀豆蛋白A（Con A）后增殖的能力。预先接受冷冻手术3天的荷瘤（t.b.）动物的脾脏由于大量高度增殖的成红细胞的产生而出现明显的脾肿大。成红细胞不太可能是抑制作用的来源，因为时间进程研究表明，在它们出现在脾脏之前和之后都存在抑制细胞。因此，成红细胞可能反映了宿主对手术期间丢失的红细胞进行再生的反应，并且它们的存在与抑制细胞的出现无关。对抑制细胞的特性分析表明，它不具有黏附性且酯酶呈阴性，因此不太可能属于巨噬细胞（MO）谱系。来自t.b.冷冻手术治疗动物第3天的脾脏MO能够在MO耗尽后完全恢复Con A依赖的T淋巴细胞增殖，这证实了上述结论。由于尼龙毛柱洗脱细胞能够抑制Con A依赖的T淋巴细胞增殖，B淋巴细胞似乎不太可能在冷冻手术诱导的免疫抑制中发挥作用。这些发现表明，对t.b.动物进行冷冻手术会导致一群能够抑制Con A依赖的T淋巴细胞增殖的T淋巴细胞产生，并推断与转移性肿瘤切除相比，这些细胞导致了冷冻手术后较差的预后。
