Wang B S, Heacock E H, Wu A V, Mannick J A
J Clin Invest. 1980 Aug;66(2):200-9. doi: 10.1172/JCI109845.
Immunoincompetency is often seen in patients after various types of trauma and is associated with increased morbidity and mortality from infectious complications. To understand better the immunologic impairment associated with trauma, we have studied this phenomenon in an animal model. Splenocytes from mice traumatized by amputation of their right hind limbs were consistently shown to have a diminished capacity to proliferate in response to alloantigens and to form alloreactive cytolytic cells in mixed lymphocyte cultures. Anesthesia itself had no effect in this system. The immunoincompetency was detected from 2 h to 6 d after surgical trauma and was completely reversed by removing adherent and phagocytic cells from the splenocytes. Furthermore, addition of splenocytes from traumatized mice to mixed lymphocyte cultures from normal mice prevented normal lymphocytes from responding to alloantigens, suggesting the existence of suppressor cells. The suppressor cells were found to adhere to glass and to nylon wool columns, and were contained within an esterase-positive cell population. They were insensitive to treatment with anti-Thy 1.2 and anti-Ig sera in the presence of complement. Therefore, the present results suggest that a Thy 1.2-negative, Ig-negative, esterase-positive cell population capable of adhering to glass and nylon wool, presumably macrophages, was responsible for the inhibition of the responsiveness of lymphocytes to alloantigens in traumatized animals.
免疫功能不全在各类创伤后的患者中很常见，且与感染性并发症导致的发病率和死亡率增加有关。为了更好地理解与创伤相关的免疫损伤，我们在动物模型中研究了这一现象。 consistently shown to have a diminished capacity to proliferate in response to alloantigens and to form alloreactive cytolytic cells in mixed lymphocyte cultures. 麻醉本身在该系统中没有影响。免疫功能不全在手术创伤后2小时至6天被检测到，通过从脾细胞中去除黏附细胞和吞噬细胞可使其完全恢复。此外，将创伤小鼠的脾细胞添加到正常小鼠的混合淋巴细胞培养物中，会阻止正常淋巴细胞对同种异体抗原作出反应，这表明存在抑制细胞。发现抑制细胞黏附于玻璃和尼龙毛柱，并存在于酯酶阳性细胞群体中。在补体存在的情况下，它们对用抗Thy 1.2和抗Ig血清处理不敏感。因此，目前的结果表明，一个能够黏附于玻璃和尼龙毛的Thy 1.2阴性、Ig阴性、酯酶阳性细胞群体，可能是巨噬细胞，负责抑制创伤动物淋巴细胞对同种异体抗原的反应性。
