Hasunuma K, Shinohara Y
National Institute for Basic Biology, Okazaki, Japan.
Curr Genet. 1986;10(12):893-901. doi: 10.1007/BF00398286.
Two regulatory mutants in orthophosphate-regulated cyclic phosphodiesterase (cPDase), cpd-3 and cpd-4, were isolated and mapped proximal to arg-1 on L.G. IC and distal to arg-12 on L.G. IIR, respectively. cpd-3 showed short aerial hyphae with dense formation of conidia. The morphology was very similar to that of cr-1, cpd-3 and cr-1 had reduced levels of cyclic 3',5'-AMP, adenylate cyclase and cPDases (CPDase I, II and III in low phosphate condition) but had elevated levels of cyclic 3',5'-GMP. Although cr-1 showed an enhanced level and enhanced activation of heat activated cyclic phosphodiesterase (ha-PDE), this enzyme was not activated in cpd-3. cpd-4, nuc-1 and nuc-2 produced neither cPDase I, II, III, alkaline phosphatase nor ribonuclease N1 in low phosphate media. These mutants did not produce aerial hyphae or conidia when grown in low phosphate liquid medium. Activation of ha-PDE occurred in cpd-4, but not in nuc-1 and nuc-2.
分离出正磷酸盐调节的环磷酸二酯酶（cPDase）中的两个调节突变体cpd-3和cpd-4，它们分别定位于LG IC上arg-1的近端和LG IIR上arg-12的远端。cpd-3表现出短的气生菌丝，分生孢子密集形成。其形态与cr-1非常相似，cpd-3和cr-1的环3',5'-AMP、腺苷酸环化酶和cPDases（低磷酸盐条件下的CPDase I、II和III）水平降低，但环3',5'-GMP水平升高。尽管cr-1显示热激活环磷酸二酯酶（ha-PDE）的水平增强和激活增强，但该酶在cpd-3中未被激活。cpd-4、nuc-1和nuc-2在低磷酸盐培养基中既不产生cPDase I、II、III、碱性磷酸酶，也不产生核糖核酸酶N1。这些突变体在低磷酸盐液体培养基中生长时不产生气生菌丝或分生孢子。ha-PDE的激活发生在cpd-4中，但在nuc-1和nuc-2中未发生。
