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粗糙脉孢菌野生型细胞和无功能型突变体在培养基中分泌的π-可阻遏酶的特性分析。

Characterization of Pi-repressible enzymes secreted in culture media by Neurospora crassa wild-type cells and null-type mutants.

作者信息

Furukawa K, Hasunuma K, Shinohara Y

机构信息

National Institute for Basic Biology, Okazaki, Japan.

出版信息

J Bacteriol. 1987 Oct;169(10):4790-5. doi: 10.1128/jb.169.10.4790-4795.1987.

DOI:10.1128/jb.169.10.4790-4795.1987
PMID:2820943
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC213856/
Abstract

In wild-type mycelial cultures of Neurospora crassa under Pi-limited conditions, alkaline phosphatase, cyclic phosphodiesterases I, II, III, and IV, 5'-nucleotidase, acid and alkaline nucleases, RNase N1, and a newly detected endonuclease were secreted into the culture media. These enzymes were either not produced or were produced in very reduced levels in mutants nuc-1, -2, -3, -4, -5, -6, and -7 and cpd-4. The proteins were examined by polyacrylamide gel electrophoresis in a manner which allowed the identification of each of them.

摘要

在粗糙脉孢菌野生型菌丝体培养物中，在磷限制条件下，碱性磷酸酶、环磷酸二酯酶I、II、III和IV、5'-核苷酸酶、酸性和碱性核酸酶、核糖核酸酶N1以及一种新检测到的内切核酸酶被分泌到培养基中。在nuc-1、-2、-3、-4、-5、-6和-7以及cpd-4突变体中，这些酶要么不产生，要么产生水平极低。通过聚丙烯酰胺凝胶电泳对这些蛋白质进行检测，以便能够鉴定出每一种蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b58/213856/5ac01c442433/jbacter00200-0388-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b58/213856/ba521fc7ab41/jbacter00200-0387-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b58/213856/3597b7549b64/jbacter00200-0387-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b58/213856/5ac01c442433/jbacter00200-0388-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b58/213856/ba521fc7ab41/jbacter00200-0387-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b58/213856/3597b7549b64/jbacter00200-0387-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b58/213856/5ac01c442433/jbacter00200-0388-a.jpg

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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
PHO-regulon of Escherichia coli K12: a minireview.大肠杆菌K12的PHO调节子：一篇综述
Ann Microbiol (Paris). 1982 Mar-Apr;133(2):243-9.
3
Phosphate-controlled gene expression in Escherichia coli K12 using Mudl-directed lacZ fusions.利用Mudl定向的lacZ融合技术研究大肠杆菌K12中磷酸盐控制的基因表达。
J Mol Biol. 1982 Jul 5;158(3):347-63. doi: 10.1016/0022-2836(82)90202-9.
4
Nucleases and their control in wild-type and nuh mutants of Neurospora.
Basic Life Sci. 1980;15:63-74. doi: 10.1007/978-1-4684-3842-0_5.
5
How the lambda repressor and cro work.λ阻遏蛋白和Cro蛋白是如何发挥作用的。
Cell. 1980 Jan;19(1):1-11. doi: 10.1016/0092-8674(80)90383-9.
6
Repressible extracellular phosphodiesterases showing cyclic 2',3'- and cyclic 3',5'-nucleotide phosphodiesterase activities in Neurospora crassa.在粗糙脉孢菌中表现出2',3'-环核苷酸和3',5'-环核苷酸磷酸二酯酶活性的可阻遏细胞外磷酸二酯酶。
J Bacteriol. 1983 Oct;156(1):291-300. doi: 10.1128/jb.156.1.291-300.1983.
7
Biochemical genetics of Neurospora nuclease II: Mutagen sensitivity and other characteristics of the nuclease mutants.
Genet Res. 1983 Jun;41(3):287-97. doi: 10.1017/s0016672300021340.
8
Biochemical genetics of Neurospora nuclease I: Isolation and characterization of nuclease (nuc) mutants.
Genet Res. 1983 Jun;41(3):271-86. doi: 10.1017/s0016672300021339.
9
Regulation of a Neurospora crassa extracellular RNase by phosphorus, nitrogen, and carbon derepressions.粗糙脉孢菌胞外核糖核酸酶受磷、氮和碳源去阻遏的调控。
J Bacteriol. 1984 Feb;157(2):380-4. doi: 10.1128/jb.157.2.380-384.1984.
10
Characterization and comparison of a Neurospora crassa RNase purified from cultures undergoing each of three different states of derepression.对从经历三种不同去阻遏状态的培养物中纯化得到的粗糙脉孢菌核糖核酸酶的特性分析与比较。
J Bacteriol. 1984 Feb;157(2):375-9. doi: 10.1128/jb.157.2.375-379.1984.