通过重组载体治愈酵母中的内源2微米DNA
Curing of endogenous 2 micron DNA in yeast by recombinant vectors.
作者信息
Harford M N, Peeters M
机构信息
Molecular Genetics Department, Smith Kline-RIT, Rixensart, Belgium.
出版信息
Curr Genet. 1987;11(4):315-9. doi: 10.1007/BF00355406.
PMID:2834096
Abstract
Passaging on selective media of yeast strains transformed with complete 2 micron vectors carrying TRP1, LEU2 or URA3 selective markers leads to curing of the resident endogenous 2 micron DNA in a majority of the population. Vector plasmids defective in FLP function are fixed as populations of A, A + B or B forms after 2 micron loss. Transformation with these plasmids offers a general method of obtaining cir degree derivatives of any yeast strain.
摘要
用携带TRP1、LEU2或URA3选择标记的完整2微米载体转化酵母菌株后，在选择性培养基上传代培养会导致大多数群体中的内源性2微米DNA被清除。FLP功能有缺陷的载体质粒在2微米DNA丢失后会以A、A + B或B形式固定下来。用这些质粒进行转化提供了一种获得任何酵母菌株cir度衍生物的通用方法。
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