酵母中的转化：一种杂种克隆载体的构建及CAN1基因的分离
Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.
作者信息
Broach J R, Strathern J N, Hicks J B
出版信息
Gene. 1979 Dec;8(1):121-33. doi: 10.1016/0378-1119(79)90012-x.
PMID:395029
Abstract
We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2 mu circule. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.
摘要
我们构建了一种质粒YEp13，当它与酵母转化结合使用时，是分离特定酵母基因的合适载体。该质粒由pBR322、酵母的LEU2基因以及一个包含来自2μm质粒的酵母复制起点的DNA片段组成。我们通过从插入YEp13的随机酵母DNA片段库中分离编码精氨酸通透酶的酵母基因CAN1，证明了这个克隆系统的实用性。
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