Girard-Bascou J, Choquet Y, Schneider M, Delosme M, Dron M
Institut de Biologie Physico-Chimique, Paris, France.
Curr Genet. 1987;12(7):489-95. doi: 10.1007/BF00419557.
The synthesis of polypeptides related to the CPI chlorophyll-protein complex of photosystem I has been studied by pulse-labeling experiments in twenty chloroplast mutants of Chlamydomonas reinhardtii. Three mutations of the same locus (Girard-Bascou 1987) result in the absence of these CPI-related polypeptides. Among these mutations one, (FUD26) leads to the synthesis of a new polypeptide presumed to be a truncated CPI apoprotein. The molecular characterization of this mutation in the psaA2 gene has been achieved by DNA sequencing the 3' end of this gene. The FUD26 mutation is a 4 base pair deletion resulting in frameshift and premature termination of the protein.
通过对莱茵衣藻20个叶绿体突变体进行脉冲标记实验，研究了与光系统I的CPI叶绿素-蛋白复合体相关的多肽合成。同一基因座的三个突变（吉拉尔-巴斯库，1987年）导致这些与CPI相关的多肽缺失。在这些突变中，一个突变（FUD26）导致合成一种新的多肽，推测为截短的CPI脱辅基蛋白。通过对该基因3'端进行DNA测序，实现了对psaA2基因中该突变的分子特征分析。FUD26突变是一个4碱基对的缺失，导致蛋白质移码和提前终止。
