Kitada K, Hishinuma F
Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
Curr Genet. 1988;13(1):1-5. doi: 10.1007/BF00365748.
We have determined DNA sequences of the mating factor alpha genes of Saccharomyces uvarum and Saccharomyces italicus and compared them to that of the MF alpha 1 gene of S. cerevisiae. The DNA sequences of the mating factor genes in both species were almost completely identical to that of the MF alpha 1 gene of S. cerevisiae except for the number of tandem repeated units; these latter consisted of a spacer peptide and a mature mating factor and there were three units in S. uvarum and five units in S. italicus compared with four units in the MF alpha 1 of S. cerevisiae. From the detailed comparison of DNA sequences of the spacer peptide-mating factor units from these three species, the high sequence homology can be recognized in the internal units of the tandem repeats. This suggests that the internal units might be multiplied preferentially in the tandem repeated units of mating factor genes.
我们已经测定了葡萄汁酵母和意大利酵母交配因子α基因的DNA序列，并将它们与酿酒酵母的MFα1基因的序列进行了比较。这两个物种中交配因子基因的DNA序列，除了串联重复单元的数量外，几乎与酿酒酵母的MFα1基因的序列完全相同；后者由一个间隔肽和一个成熟的交配因子组成，葡萄汁酵母中有三个单元，意大利酵母中有五个单元，而酿酒酵母的MFα1中有四个单元。通过对这三个物种间隔肽-交配因子单元的DNA序列进行详细比较，可以发现在串联重复序列的内部单元中存在高度的序列同源性。这表明内部单元可能在交配因子基因的串联重复单元中优先倍增。
