Selakovic Dragica, Joksimovic Jovana, Obradovic Dragan, Milovanovic Dragan, Djuric Milos, Rosic Gvozden
Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Serbia.
Institute of Pharmacology, Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Belgrade, Serbia.
Neuro Endocrinol Lett. 2016 Oct;37(5):383-388.
The aim of this study was the estimation of effects induced by chronic administration of supraphysiological dose of TE and prolonged exercise in male rats on anxiety levels (alterations in exploratory activity patterns in EPM).
Two sedentary (control - C and testosterone-enanthate - T) and two exercise (exercise - E and testosterone-enanthate plus exercise - T+E) groups (n=32) underwent adequate protocols - the swimming protocol (1 h/day) and TE (20 mg/kg/w, s.c.) for six weeks. Testing was performed in EPM.
Anxiolytic effects of exercise were manifested as increased exploratory activity in EPM - increase in cumulative duration in open arms, the number of rearings and head-dippings, and TEA. Supraphysiological dose of TE decreased the number of rearings and head-dippings, cumulative duration in open arms and TEA compared to the control group, while this effect of TE was more pronounced compared to the exercise group. The applied dose of TE attenuated beneficial effects of exercise by means of all estimated parameters.
Our results confirmed the beneficial effect of exercise on anxiety levels observed in EPM by means of parameters considering the alterations in exploratory activity. Supraphysiological dose of TE resulted in anxiogenic-like behavior in EPM. The effect of TE was so pronounced that the beneficial effect of exercise was reversed to the control values (or even below them). Based on the results of this trial, we propose that use of the TEA (a new parameter for overall exploratory activity) can improve the evaluation of EPM test results.
本研究旨在评估长期给予超生理剂量的睾酮庚酸酯（TE）以及长期运动对雄性大鼠焦虑水平（高架十字迷宫中探索活动模式的改变）的影响。
两组久坐不动的大鼠（对照组 - C 和睾酮庚酸酯组 - T）以及两组运动大鼠（运动组 - E 和睾酮庚酸酯加运动组 - T + E）（每组 n = 32）接受了为期六周的适当方案——游泳方案（每天 1 小时）和 TE（20 毫克/千克/周，皮下注射）。在高架十字迷宫中进行测试。
运动的抗焦虑作用表现为在高架十字迷宫中探索活动增加——开放臂累积持续时间增加、站立次数和探头次数增加以及总探索活动（TEA）增加。与对照组相比，超生理剂量的 TE 减少了站立次数和探头次数、开放臂累积持续时间以及 TEA，而与运动组相比，TE 的这种作用更为明显。所应用的 TE 剂量通过所有评估参数减弱了运动的有益作用。
我们的结果通过考虑探索活动改变的参数证实了运动对高架十字迷宫中观察到的焦虑水平的有益作用。超生理剂量的 TE 在高架十字迷宫中导致类似焦虑的行为。TE 的作用非常明显，以至于运动的有益作用被逆转至对照值（甚至低于对照值）。基于该试验结果，我们建议使用总探索活动（TEA，一个新的探索活动参数）可以改善对高架十字迷宫测试结果的评估。
