促性腺激素释放激素类似物在虹鳟鱼、内陆鲑鱼和冬比目鱼中的生物活性研究。

Studies of the biological activity of LHRH analogs in the rainbow trout, landlocked salmon, and the winter flounder.

作者信息

Crim L W, Nestor J J, Wilson C E

机构信息

Marine Sciences Research Laboratory, Memorial University of Newfoundland, St. John's, Canada.

出版信息

Gen Comp Endocrinol. 1988 Sep;71(3):372-82. doi: 10.1016/0016-6480(88)90265-1.

DOI:10.1016/0016-6480(88)90265-1

PMID:2847951

Abstract

Studies of gonadotropic hormone (GtH) release bioactivity by mammalian and submammalian varieties of LHRH and LHRH analog were primarily conducted in vivo in testosterone-primed yearling (TPY) rainbow trout, a convenient test animal for LHRH bioassays in fish. Validation of these results, using sexually mature fish, was accomplished by examining LHRH agonist activities on release of GtH in vivo in spermiating landlocked salmon and by studying LHRH peptide hormone binding affinities using a flounder pituitary LHRH radioreceptor assay. Our surveys of LHRH analog bioactivity in vivo in TPY trout and salmon demonstrated that all types of fish, bird, and mammalian LHRH agonists possess superactive properties on the fish pituitary. The most active group of LHRH analogs, based upon both LHRH receptor binding affinity and in vivo release of gonadotropin, was judged to include [D-Nal(2)6,Pro9-NHEt]LHRH, [D-Nal(2)6-AzaGly10]LHRH, [D-Ala6,Pro9-NHEt]-LHRH, and the fish LHRH analogs, [D-Arg6,Trp7,Leu8,Pro9-NHEt]LHRH, [D-hArg(Et2(6),Trp7,Leu8,Pro9-NHEt]LHRH.

摘要

对哺乳动物和低于哺乳动物种类的促黄体激素释放激素（LHRH）及LHRH类似物的促性腺激素（GtH）释放生物活性的研究，主要是在经睾酮预处理的一岁虹鳟鱼（TPY）体内进行的，这是鱼类LHRH生物测定中一种方便的试验动物。通过检测在成熟的内陆鲑鱼体内LHRH激动剂对GtH释放的作用，并使用比目鱼垂体LHRH放射受体测定法研究LHRH肽激素结合亲和力，从而利用性成熟鱼类对这些结果进行了验证。我们对TPY虹鳟鱼和鲑鱼体内LHRH类似物生物活性的调查表明，所有类型的鱼类、鸟类和哺乳动物LHRH激动剂对鱼类垂体均具有超活性特性。基于LHRH受体结合亲和力和促性腺激素的体内释放，最具活性的LHRH类似物组被判定包括[D - Nal(2)6,Pro9 - NHEt]LHRH、[D - Nal(2)6 - AzaGly10]LHRH、[D - Ala6,Pro9 - NHEt] - LHRH以及鱼类LHRH类似物[D - Arg6,Trp7,Leu8,Pro9 - NHEt]LHRH、[D - hArg(Et2(6),Trp7,Leu8,Pro9 - NHEt]LHRH。