Weber Florian, Barrantes Alejandro
Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, P.O. box 1109 Blindern, 0317 Oslo, Norway; Technical University of Munich, Munich, Germany.
Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, P.O. box 1109 Blindern, 0317 Oslo, Norway.
Colloids Surf B Biointerfaces. 2017 Jun 1;154:203-209. doi: 10.1016/j.colsurfb.2017.03.022. Epub 2017 Mar 9.
The formation of salivary films onto oral prostheses materials is of central importance for understanding their performance and interaction with oral tissue and flora. The aim of this work was to study and compare the salivary films formed from unstimulated and stimulated whole saliva on two common polymeric materials, polycarbonate and poly(methyl methacrylate). Irradiating these materials with UV light is a simple way to modify their wettability, roughness and ζ-potential. Therefore, the effect of UV exposure of polycarbonate and poly(methyl methacrylate) on saliva adsorption was also investigated. For this purpose a quartz crystal microbalance with dissipation and SDS-PAGE have been combined in order to associate the thicknesses and viscoelastic properties of the salivary films with their protein composition. SDS-PAGE results suggest that a larger diversity of proteins is involved in the formation of stimulated saliva pellicles. Furthermore, according to QCM-D, pellicles formed from stimulated saliva are thinner and stiffer than the ones formed from unstimulated saliva if the polymeric materials have not been exposed to UV light although both types of saliva form a biphasic layer. For UV-treated materials, the same is applied to polycarbonate but not to poly(methyl methacrylate) where stimulated saliva yields thicker and softer films than unstimulated saliva being the adsorption process of a multiphasic nature. These results highlight the importance of choosing the appropriate sample depending on the type of study to be performed.
唾液膜在口腔修复材料上的形成对于理解其性能以及与口腔组织和菌群的相互作用至关重要。本研究的目的是研究和比较由未刺激和刺激后的全唾液在两种常见聚合物材料聚碳酸酯和聚甲基丙烯酸甲酯上形成的唾液膜。用紫外线照射这些材料是改变其润湿性、粗糙度和ζ电位的一种简单方法。因此，还研究了聚碳酸酯和聚甲基丙烯酸甲酯紫外线照射对唾液吸附的影响。为此，将具有耗散功能的石英晶体微天平与十二烷基硫酸钠聚丙烯酰胺凝胶电泳相结合，以便将唾液膜的厚度和粘弹性特性与其蛋白质组成联系起来。十二烷基硫酸钠聚丙烯酰胺凝胶电泳结果表明，更多种类的蛋白质参与了刺激唾液薄膜的形成。此外，根据石英晶体微天平耗散技术，若聚合物材料未暴露于紫外线下，由刺激唾液形成的薄膜比未刺激唾液形成的薄膜更薄且更硬，尽管两种类型的唾液都形成双相层。对于经过紫外线处理的材料，聚碳酸酯情况相同，但聚甲基丙烯酸甲酯则不同，刺激唾液产生的薄膜比未刺激唾液产生的薄膜更厚且更软，吸附过程具有多相性质。这些结果凸显了根据要进行的研究类型选择合适样本的重要性。
