Russnak R H, Candido E P, Astell C R
Department of Biochemistry, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
J Biol Chem. 1988 May 5;263(13):6392-9.
High affinity mouse HMG-I binding sites have been distinguished from other (A+T)-rich sequences using band competition assays. These sites have been found 3' to the coding regions of a variety of genes. For the herpes simplex virus thymidine kinase and minute virus of mice genes, high affinity HMG-1 binding sites were further localized to the functional polyadenylation signal by DNase I footprinting. These results suggest that HMG-I may function at the 3' ends of genes in vivo.
利用条带竞争分析，已将高亲和力的小鼠HMG-I结合位点与其他富含（A+T）的序列区分开来。这些位点已在多种基因编码区的3'端被发现。对于单纯疱疹病毒胸苷激酶基因和小鼠微小病毒基因，通过DNA酶I足迹法，高亲和力的HMG-1结合位点被进一步定位到功能性聚腺苷酸化信号上。这些结果表明，HMG-I可能在体内基因的3'端发挥作用。
