GuttaFlow Bioseal、GuttaFlow2、MTA Fillapex 和 AH Plus 对人牙周膜干细胞的细胞毒性。

Cytotoxicity of GuttaFlow Bioseal, GuttaFlow2, MTA Fillapex, and AH Plus on Human Periodontal Ligament Stem Cells.

机构信息

Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, Murcia, Spain.

Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, Murcia, Spain; School of Dentistry, Faculty of Medicine, University of Murcia, Murcia, Spain.

出版信息

J Endod. 2017 May;43(5):816-822. doi: 10.1016/j.joen.2017.01.001. Epub 2017 Mar 23.

DOI:10.1016/j.joen.2017.01.001

PMID:28343929

Abstract

INTRODUCTION

The aim of the present study was to evaluate the in vitro cytotoxicity of endodontic sealers (GuttaFlow Bioseal, GuttaFlow2, and MTA Fillapex) on human periodontal ligament stem cells (hPDLSCs). As a reference, AH Plus was compared with the more recent endodontic sealers regarding cell viability and cell attachment.

METHODS

Biological testing was carried out in vitro on hPDLSCs. Cell viability assay was performed by using eluates from each endodontic sealer. To assess cell morphology and attachment to the different sealers, the hPDLSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. Chemical composition of the sealers was determined by energy-dispersive x-ray, and eluates were analyzed by inductively coupled plasma mass spectrometry. Statistical differences were assessed by analysis of variance and Tukey test (P < .05).

RESULTS

Cell viability was evident after 24 hours in the presence of GuttaFlow Bioseal and GuttaFlow 2 but not in the case of AH Plus or MTA Fillapex. At 168 hours, GuttaFlow Bioseal and GuttaFlow 2 exhibited high and moderate cell viability, respectively, whereas AH Plus and MTA Fillapex revealed low rates of cell cell viability (P < .001). Finally, scanning electron microscopy studies revealed a high degree of proliferation, cell spreading, and attachment, especially when using GuttaFlow Bioseal disks.

CONCLUSIONS

GuttaFlow Bioseal and GuttaFlow2 showed lower cytotoxicity than MTA Fillapex and AH plus. Further in vitro and in vivo investigations are required to confirm the suitability of GuttaFlow Bioseal for clinical application.

摘要

简介

本研究旨在评估根管封闭剂（GuttaFlow Bioseal、GuttaFlow2 和 MTA Fillapex）对人牙周膜干细胞（hPDLSCs）的体外细胞毒性。作为参考，将 AH Plus 与最近的根管封闭剂进行比较，以评估细胞活力和细胞黏附性。

方法

在体外对 hPDLSCs 进行生物测试。通过使用每种根管封闭剂的浸提液来进行细胞活力测定。为了评估 hPDLSCs 对不同封闭剂的形态和附着情况，将 hPDLSCs 直接接种到材料表面，并用扫描电子显微镜进行分析。通过能量色散 X 射线确定封闭剂的化学成分，并通过电感耦合等离子体质谱分析浸提液。通过方差分析和 Tukey 检验（P <.05）评估统计学差异。

结果

在存在 GuttaFlow Bioseal 和 GuttaFlow 2 的情况下，细胞活力在 24 小时后明显，而在 AH Plus 或 MTA Fillapex 的情况下则不然。在 168 小时时，GuttaFlow Bioseal 和 GuttaFlow 2 分别表现出高和中等细胞活力，而 AH Plus 和 MTA Fillapex 则显示出低的细胞活力（P <.001）。最后，扫描电子显微镜研究显示，特别是使用 GuttaFlow Bioseal 盘时，细胞增殖、细胞扩散和附着程度很高。