Biochemistry Unit, Department of Biomedical and Neuromotor Sciences, University of Bologna, Via Irnerio 48, 40126 Bologna, Italy.
Endodontic Clinical Section, Unit of Odontostomatological Sciences, Department of Biomedical and Neuromotor Sciences, University of Bologna, Via San Vitale 59, 40136 Bologna, Italy.
Molecules. 2023 Oct 14;28(20):7088. doi: 10.3390/molecules28207088.
Guttapercha (GP, trans-1,4-polyisoprene) is the most used tooth root filling material, and it must be used with an appropriate cement (typically a polydimethylsiloxane (PDMS)-based sealer) to ensure an adequate canal obturation. This study aimed to assess the bioactivity and dentin remineralization ability of a bioglass containing PDMS commercial endodontic sealer, BG-PDMS (GuttaFlow Bioseal), and to evaluate the possible influence of a GP cone (Roeko GP point) on the mineralization process. To this end, BG-PDMS disks were aged alone or in the presence of a GP cone in Hank's Balanced Salt Solution (28 d, 37 °C). Dentin remineralization experiments were carried out under the same conditions. Micro-Raman and IR analyses demonstrated that BG-PDMS is bioactive, thanks to the formation of a silica-rich layer with nucleation sites for B-type carbonated apatite deposition. This phase was thicker when BG-PDMS was aged in the presence of GP. The two materials influenced each other because GP, which alone did not show any bioactivity, nucleated a calcium phosphate phase under these conditions. Analogously, dentin remineralization experiments showed that BG-PDMS is able to remineralize dentin, especially in the presence of GP. Under the experimental conditions, GP acted as a templating agent for calcium phosphate deposition.
古塔胶（GP，反式-1,4-聚异戊二烯）是最常用的牙根管填充材料，必须与适当的水泥（通常是聚二甲基硅氧烷（PDMS）基密封剂）一起使用，以确保充分的根管封闭。本研究旨在评估含有 PDMS 的生物玻璃商业根管封闭剂 BG-PDMS（GuttaFlow Bioseal）的生物活性和牙本质再矿化能力，并评估 GP 牙胶尖（Roeko GP point）对矿化过程的可能影响。为此，将 BG-PDMS 圆盘单独或在 Hank's 平衡盐溶液中存在 GP 牙胶尖的情况下进行老化（28 d，37°C）。在相同条件下进行牙本质再矿化实验。微拉曼和红外分析表明，BG-PDMS 是具有生物活性的，这要归功于形成了一层富含硅的层，为 B 型碳酸磷灰石的沉积提供了成核点。当 BG-PDMS 在 GP 的存在下老化时，该相更厚。这两种材料相互影响，因为 GP 本身没有显示出任何生物活性，但在这些条件下会引发磷酸钙相的形成。类似地，牙本质再矿化实验表明，BG-PDMS 能够再矿化牙本质，尤其是在存在 GP 的情况下。在实验条件下，GP 充当了磷酸钙沉积的模板剂。
