通过蛋白质组学和免疫组织化学鉴定出的OLFM4、KNG1和Sec24C作为早期结直肠癌阶段的潜在标志物。
OLFM4, KNG1 and Sec24C identified by proteomics and immunohistochemistry as potential markers of early colorectal cancer stages.
作者信息
Quesada-Calvo Florence, Massot Charlotte, Bertrand Virginie, Longuespée Rémi, Blétard Noëlla, Somja Joan, Mazzucchelli Gabriel, Smargiasso Nicolas, Baiwir Dominique, De Pauw-Gillet Marie-Claire, Delvenne Philippe, Malaise Michel, Coimbra Marques Carla, Polus Marc, De Pauw Edwin, Meuwis Marie-Alice, Louis Edouard
机构信息
Gastroenterology Department, GIGA-R, Liège University Hospital CHU, ULg, GIGA CHU-B34 Avenue de l'Hôpital 11, 4000 Liège, Belgium.
Laboratory of Mass Spectrometry, Chemistry Department, GIGA-R, CART, ULg, 4000 Liège, Belgium.
出版信息
Clin Proteomics. 2017 Mar 24;14:9. doi: 10.1186/s12014-017-9143-3. eCollection 2017.
BACKGROUND
Despite recent advances in colorectal cancer (CRC) diagnosis and population screening programs, the identification of patients with preneoplastic lesions or with early CRC stages remains challenging and is important for reducing CRC incidence and increasing patient's survival.
METHODS
We analysed 76 colorectal tissue samples originated from early CRC stages, normal or inflamed mucosa by -. The characterisation of three selected biomarker candidates was performed by immunohistochemistry on an independent set of precancerous and cancerous lesions harbouring increasing CRC stages.
RESULTS
Out of 5258 proteins identified, we obtained 561 proteins with a significant differential distribution among groups of patients and controls. KNG1, OLFM4 and Sec24C distributions were validated in tissues and showed different expression levels especially in the two early CRC stages compared to normal and preneoplastic tissues.
CONCLUSION
We highlighted three proteins that require further investigations to better characterise their role in early CRC carcinogenesis and their potential as early CRC markers.
背景
尽管近年来在结直肠癌（CRC）诊断和人群筛查项目方面取得了进展，但识别癌前病变或早期CRC阶段的患者仍然具有挑战性，这对于降低CRC发病率和提高患者生存率至关重要。
方法
我们通过-分析了76份来自早期CRC阶段、正常或炎症黏膜的结直肠组织样本。对一组独立的、处于不同CRC阶段的癌前和癌性病变进行免疫组织化学分析，以鉴定三种选定的生物标志物候选物。
结果
在鉴定出的5258种蛋白质中，我们获得了561种在患者组和对照组之间具有显著差异分布的蛋白质。KNG1、OLFM4和Sec24C的分布在组织中得到验证，并且与正常和癌前组织相比，在两个早期CRC阶段显示出不同的表达水平。
结论
我们突出了三种蛋白质，需要进一步研究以更好地确定它们在早期CRC致癌过程中的作用及其作为早期CRC标志物的潜力。
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