Shahani Toran, Makvandi Manoochehr, Samarbafzadeh Alireza, Teimoori Ali, Ranjbar Nastran, saki Nader, Nikakhlagh Soheila, Neisi Niloofar, Hosseini Zeinab, Pourrezaei Samira, Shabani Abdolnabi, Radmehr Hashem, Mehravaran Hamide, Kiani Hadis, Haghi Azadeh
Health Research Institute, Infectious and Tropical Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Email:
Asian Pac J Cancer Prev. 2017 Feb 1;18(2):327-331. doi: 10.22034/APJCP.2017.18.2.327.
Background: Around 95% of the world’s population are infected with the Epstein-Barr virus (EBV), which can persist latent in B lymphocytes and epithelial cells life-long. EBV has been linked with lymphoid and epithelial cancers and persistence of EBV infection in lymphoid or epithelial cells may result in virus-associated B-cell tumors or nasopharyngeal carcinomas (NPC). This study was conducted to determine the frequency of EBV DNA in nasopharyngeal carcinoma tissue of Iranian patients. Materials and methods: A total of 50 blocks of formalin-fixed paraffin-embedded tissue of NPCs from 38 (76 %) male and 12 (24%) female patients were collected from archives of Ahvaz hospitals. Sections were cut at 5 μm and DNA was extracted for detection of EBV DNA and EBV typing by mested PCR. DNA sequencing was performed to confirm PCR results. The distribution of EBV DNA was compared among WHO histological subtypes of NPC. Results: Some 3 female and 11 (22%) male NPC samples showed positive for EBV DNA type 1, 2/14(22.2%)WHO histological type II and 12/41(29.3%) WHO histological type III. Conclusions: The frequency of EBV DNA among NPCs in Iranian patients was found to be 28%, EBV type I predominating. Both WHO histological type II and III NPC subtypes demonstrated approximately the same detection prevalence.
全球约95%的人口感染过爱泼斯坦-巴尔病毒（EBV），该病毒可在B淋巴细胞和上皮细胞中终身潜伏。EBV与淋巴癌和上皮癌有关，EBV在淋巴或上皮细胞中的持续感染可能导致病毒相关的B细胞肿瘤或鼻咽癌（NPC）。本研究旨在确定伊朗患者鼻咽癌组织中EBV DNA的频率。
从阿瓦士医院的档案中收集了50个经福尔马林固定、石蜡包埋的鼻咽癌组织块，这些组织来自38名（76%）男性和12名（24%）女性患者。切片厚度为5μm，提取DNA用于通过巢式PCR检测EBV DNA和EBV分型。进行DNA测序以确认PCR结果。比较了NPC的WHO组织学亚型中EBV DNA的分布情况。
约3名女性和11名（22%）男性NPC样本的EBV DNA 1型呈阳性，WHO组织学II型中有2/14（22.2%）呈阳性，WHO组织学III型中有12/41（29.3%）呈阳性。
发现伊朗患者鼻咽癌中EBV DNA的频率为28%，以I型为主。WHO组织学II型和III型NPC亚型的检测患病率大致相同。
