Lee Yu Geon, Cho Jeong-Yong, Hwang Eom Ji, Jeon Tae-Il, Moon Jae-Hak
a Department of Food Science and Technology and Functional Food Research Center, BK21 Plus Program , Chonnam National University , Gwangju , South Korea.
b Bioenergy Crop Research Center , National Institute of Crop Science, Rural Development Administration , Muan , South Korea.
Biosci Biotechnol Biochem. 2017 Jul;81(7):1409-1416. doi: 10.1080/09168451.2017.1303358. Epub 2017 Mar 27.
A Glu-Phe (EF) was isolated from onion (Allium cepa L. cv. Sunpower). The chemical structure of EF was determined by nuclear magnetic resonance and electrospray ionization-mass (ESI-MS) spectroscopy. We showed that EF reduced lipid accumulation in mouse hepatocytes by inhibiting the expression of sterol regulatory element-binding protein-1c (SREBP-1c) and its lipogenic target genes. We also found that AMP-activated protein kinase (AMPK) was required for the inhibitory effect of EF on lipid accumulation in mouse hepatocytes. Furthermore, EF was qualified in nine onion cultivars by selective multiple reaction-monitoring detection of liquid chromatography-ESI-MS. These results suggest that EF could contribute to the beneficial effect of onion supplement in maintaining hepatic lipid homeostasis.
从洋葱（Allium cepa L. cv. Sunpower）中分离出一种谷氨酰-苯丙氨酸（EF）。通过核磁共振和电喷雾电离质谱（ESI-MS）光谱确定了EF的化学结构。我们发现EF通过抑制固醇调节元件结合蛋白-1c（SREBP-1c）及其脂肪生成靶基因的表达来减少小鼠肝细胞中的脂质积累。我们还发现，EF对小鼠肝细胞脂质积累的抑制作用需要AMP激活的蛋白激酶（AMPK）。此外，通过液相色谱-ESI-MS的选择性多反应监测检测，在九个洋葱品种中鉴定了EF。这些结果表明，EF可能有助于洋葱补充剂在维持肝脏脂质稳态方面的有益作用。
