Department of Biomolecular Sciences, Weizmann Institute of Science , Rehovot 7610001, Israel.
Anal Chem. 2017 Apr 18;89(8):4398-4404. doi: 10.1021/acs.analchem.7b00398. Epub 2017 Apr 5.
Determining the properties of proteins prior to purification saves time and labor. Here, we demonstrate a native mass spectrometry approach for rapid characterization of overexpressed proteins directly in crude cell lysates. The method provides immediate information on the identity, solubility, oligomeric state, overall structure, and stability, as well as ligand binding, without the need for purification.
在进行蛋白质纯化之前确定其性质可以节省时间和劳动力。在这里，我们展示了一种用于快速鉴定粗提细胞裂解物中表达的蛋白质的质谱方法。该方法可以直接提供有关蛋白质的身份、可溶性、寡聚状态、整体结构和稳定性以及配体结合的信息，而无需进行纯化。
