构巢曲霉乙醇利用调节子的正调控基因alcR的调控
Regulation of alcR, the positive regulatory gene of the ethanol utilization regulon of Aspergillus nidulans.
作者信息
Lockington R, Scazzocchio C, Sequeval D, Mathieu M, Felenbok B
机构信息
Institut de Microbiologie, Université Paris-Sud, Orsay, France.
出版信息
Mol Microbiol. 1987 Nov;1(3):275-81. doi: 10.1111/j.1365-2958.1987.tb01933.x.
The alcR positive control gene is necessary for the expression of both alcA (coding for alcohol dehydrogenase ADH I), and aldA (coding for aldehyde dehydrogenase, AldDH) in Aspergillus nidulans. Using a cloned alcR probe and Northern blots analysis we show that: (1) alcR itself is inducible; (2) alcR inducibility depends on the expression of the alcR gene itself; and (3) alcR is subject to carbon catabolite repression and its expression is controlled by the negatively acting creA wide specificity gene. The repression of alcR is sufficient to explain the carbon catabolite repression of ADH I and AldDH.
在构巢曲霉中，alcR阳性对照基因对于alcA（编码乙醇脱氢酶ADH I）和aldA（编码醛脱氢酶AldDH）的表达都是必需的。利用克隆的alcR探针和Northern印迹分析，我们发现：（1）alcR自身是可诱导的；（2）alcR的可诱导性取决于alcR基因自身的表达；（3）alcR受到碳分解代谢物阻遏，其表达受负向作用的creA广泛特异性基因控制。alcR的阻遏足以解释ADH I和AldDH的碳分解代谢物阻遏现象。
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