Ghasemikhah Reza, Tabatabaiefar Mohammad Amin, Shariatzadeh Seyed Ali, Shahbazi Abbas, Hazratian Teymour
Department of Parasitology& Mycology, School of Medicine, Arak University of Medical Sciences, Arak 3848176941, Iran.
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan 8174673461, Iran.
Sci Pharm. 2017 Mar 27;85(2):17. doi: 10.3390/scipharm85020017.
is a nematode causing serious infections in immunocompromised patients. In chronically infected patients, the low parasitic content as well as the resemblance of the larvae to several other species make diagnosis basedonmorphology difficult. In the present study, a PCR-based method targeting the internal transcribed sequence 2 (ITS2) of the rDNA region was examined for the molecular detection of infection from the stool samples. A total of 1800 patients were included. Three fresh stool samples were collected per patient, and isolates were identified by the morphological method. A subset of isolates was later used in the PCR-based method as positive controls. Additionally, negative and no-template controls were included. Data analysis was accomplished using an test. A-value less than 0.05 was considered significant. In total, fivestool samples were found to be infected with using the morphology method. PCR method detected DNA target from all of the fiveDNA samples extracted from positive fecal samples.
The PCR method used for amplifying a short fragment was successful for diagnosis of in fecal samples and can be reliable for directly detecting the parasite bypassing morphological method.
是一种可在免疫功能低下患者中引起严重感染的线虫。在慢性感染患者中，寄生虫含量低以及幼虫与其他几种物种相似，使得基于形态学的诊断变得困难。在本研究中，检测了一种针对核糖体DNA区域内转录间隔区2（ITS2）的基于聚合酶链反应（PCR）的方法，用于从粪便样本中分子检测感染情况。总共纳入了1800名患者。每位患者收集三份新鲜粪便样本，并通过形态学方法鉴定分离株。随后，一部分分离株被用作基于PCR方法的阳性对照。此外，还包括阴性对照和无模板对照。使用检验进行数据分析。A值小于0.05被认为具有统计学意义。使用形态学方法总共发现五份粪便样本感染了。PCR方法从所有五份从阳性粪便样本中提取的DNA样本中检测到了DNA靶点。
用于扩增短片段的PCR方法成功用于粪便样本中感染的诊断，并且可以可靠地直接检测寄生虫，绕过形态学方法。
