Hepburn A, Dumont J E
Institut de Recherche Interdisciplinaire, Faculty of Medicine, Free University of Brussels, Campus Erasme, Belgium.
Mutat Res. 1988 May;199(1):221-8. doi: 10.1016/0027-5107(88)90249-7.
The effect of increased cellular concentrations of adenosine 3',5' monophosphate (cAMP) upon mutation frequency induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was studied in V79 Chinese hamster lung cells. Incubation with either forskolin, which increased the accumulation of cAMP, or 8BrcAMP, an analogue of cAMP, resulted in an increase in the mutation frequency which was concentration-dependent, regardless of whether these agents were added before or after mutagen treatment. Increased cAMP concentrations were shown in these cells to inhibit growth; however, this does not seem to be the mechanism responsible for the increase in mutation frequency as low serum concentrations which also retard growth reduced the mutation frequency observed with MNNG.
在V79中国仓鼠肺细胞中研究了细胞内3',5'-环磷酸腺苷（cAMP）浓度升高对N-甲基-N'-硝基-N-亚硝基胍（MNNG）诱导的突变频率的影响。用可增加cAMP积累的福斯高林或cAMP类似物8-溴环磷腺苷（8BrcAMP）孵育，无论这些试剂是在诱变处理之前还是之后添加，都会导致突变频率增加，且呈浓度依赖性。在这些细胞中显示，cAMP浓度升高会抑制生长；然而，这似乎不是导致突变频率增加的机制，因为低血清浓度也会阻碍生长，但却降低了MNNG观察到的突变频率。
