Rebeyrotte N
Mutat Res. 1983 Mar;108(1-3):57-66. doi: 10.1016/0027-5107(83)90109-4.
Micrococcus radiodurans was highly resistant to the lethal effect of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) but it was sensitive to the mutagenic action of this chemical. The induction of mutation was not significantly modified by the culture growth phase. This last finding leads to the assumption that the mutation takes place at some distance from the replication fork. Moreover, a low concentration of MNNG induced mutations that were added to those subsequently obtained from a second exposure to a higher concentration of the alkylating agent. Thus, M. radiodurans does not seem to have an inducible error-free repair system for alkylation damage. Furthermore, incubation in the presence of chloramphenicol did not modify the mutation rate, indicating that protein synthesis is not involved in the mutagenic process.
耐辐射微球菌对N-甲基-N'-硝基-N-亚硝基胍（MNNG）的致死作用具有高度抗性，但对这种化学物质的诱变作用敏感。培养生长阶段对突变的诱导没有显著影响。这一最新发现导致这样一种假设，即突变发生在与复制叉有一定距离的地方。此外，低浓度的MNNG诱导的突变会叠加在随后第二次暴露于较高浓度烷基化剂所产生的突变之上。因此，耐辐射微球菌似乎没有针对烷基化损伤的可诱导无差错修复系统。此外，在氯霉素存在下孵育并没有改变突变率，这表明蛋白质合成不参与诱变过程。
