Stankevicins L, Barat A, Dessen P, Vassetzky Y, de Moura Gallo C V
Departamento de Genética, Universidade do Estado do Rio de Janeiro, Instituto de Biologia Roberto Alcantara Gomes, Rio de Janeiro, Brazil.
CNRS UMR 8126 «Signalisation, noyaux et innovations en cancérologie», Université Paris-Sud, Institut de Cancérologie Gustave-Roussy, Villejuif cedex, France.
PLoS One. 2017 Mar 27;12(3):e0173756. doi: 10.1371/journal.pone.0173756. eCollection 2017.
MicroRNA is a class of noncoding RNAs able to base pair with complementary messenger RNA sequences, inhibiting their expression. These regulatory molecules play important roles in key cellular processes including cell proliferation, differentiation and response to DNA damage; changes in miRNA expression are a common feature of human cancers. To gain insights into the mechanisms involved in breast cancer progression we conducted a microRNA global expression analysis on a 21T series of cell lines obtained from the same patient during different stages of breast cancer progression. These stages are represented by cell lines derived from normal epithelial (H16N2), atypical ductal hyperplasia (21PT), primary in situ ductal carcinoma (21NT) and pleural effusion of a lung metastasis (21MT-1 and 21MT-2). In a global microRNA expression analysis, miR-205-5p was the only miRNA to display an important downregulation in the metastatic cell lines (21MT-1; 21MT-2) when compared to the non-invasive cells (21PT and 21NT). The lower amounts of miR-205-5p found also correlated with high histological grades biopsies and with higher invasion rates in a Boyden chamber assay. This work pinpoints miR-205-5p as a potential player in breast tumor invasiveness.
微小RNA是一类非编码RNA，能够与互补的信使RNA序列进行碱基配对，从而抑制其表达。这些调节分子在包括细胞增殖、分化和对DNA损伤的反应等关键细胞过程中发挥重要作用；微小RNA表达的变化是人类癌症的一个共同特征。为了深入了解乳腺癌进展所涉及的机制，我们对从同一患者乳腺癌进展不同阶段获得的21T系列细胞系进行了微小RNA全局表达分析。这些阶段由源自正常上皮细胞（H16N2）、非典型导管增生（21PT）、原发性原位导管癌（21NT）以及肺转移胸腔积液（21MT - 1和21MT - 2）的细胞系代表。在全局微小RNA表达分析中，与非侵袭性细胞（21PT和21NT）相比，miR - 205 - 5p是唯一在转移性细胞系（21MT - 1；21MT - 2）中显示出显著下调的微小RNA。在活检中发现的较低水平的miR - 205 - 5p也与高组织学分级以及在博伊登室试验中的较高侵袭率相关。这项工作将miR - 205 - 5p确定为乳腺癌侵袭性中的一个潜在因素。
