Zeng Xianchun, Wang Nanzhu, Zhai Hui, Wang Rongpin, Wu Jiahong, Pu Wei
1 Department of Medical Imaging, Guizhou Provincial People's Hospital, Guiyang, China.
2 School of Basic Medicine, Guizhou Medical University, Guiyang, China.
Tumour Biol. 2017 Mar;39(3):1010428317691178. doi: 10.1177/1010428317691178.
Hepatocellular carcinoma is one of the leading causes for cancer-related mortality worldwide. SIRT3 may function as either oncogene or tumor suppressor in a panel of cancers; however, the role of SIRT3 in hepatocellular carcinoma remains unclear. In this study, we assayed the expression level of SIRT3 in hepatocellular carcinoma tissues by quantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry. A loss-of-function approach was used to examine the effects of SIRT3 on biological activity, including cell proliferative activity and invasive potential. The results demonstrated that the expression levels of SIRT3 protein in hepatocellular carcinoma tissues were significantly downregulated compared with those in adjacent non-cancerous tissues. Furthermore, SIRT3 could decrease cell proliferation and inhibit cell migration/invasion in hepatocellular carcinoma cell line. Taken together, these results elucidated the function of SIRT3 in hepatocellular carcinoma development and suggested that SIRT3 might function as tumor suppressor in hepatocellular carcinoma by targeting PI3K/Akt pathway.
肝细胞癌是全球癌症相关死亡的主要原因之一。SIRT3在一系列癌症中可能作为癌基因或肿瘤抑制因子发挥作用；然而，SIRT3在肝细胞癌中的作用仍不清楚。在本研究中，我们通过定量逆转录 - 聚合酶链反应和免疫组织化学检测了肝细胞癌组织中SIRT3的表达水平。采用功能丧失方法来研究SIRT3对生物学活性的影响，包括细胞增殖活性和侵袭潜能。结果表明，与相邻的非癌组织相比，肝细胞癌组织中SIRT3蛋白的表达水平显著下调。此外，SIRT3可降低肝细胞癌细胞系中的细胞增殖并抑制细胞迁移/侵袭。综上所述，这些结果阐明了SIRT3在肝细胞癌发生发展中的功能，并表明SIRT3可能通过靶向PI3K/Akt通路在肝细胞癌中发挥肿瘤抑制作用。
