Department of Bioengineering, Rice University, Houston, Texas 77251.
Department of Chemical and Biomolecular Engineering, Rice University, Houston, Texas 77251.
Cold Spring Harb Perspect Med. 2017 Dec 1;7(12):a025734. doi: 10.1101/cshperspect.a025734.
An avenue of tremendous interest and need in health care encompasses the regeneration of bone and cartilage. Over the years, numerous tissue engineering strategies have contributed substantial progress toward the realization of clinically relevant therapies. Cell and tissue culture protocols, however, show many variations that make experimental results among different publications challenging to compare. This collection surveys prevalent cell sources, soluble factors, culture medium formulations, environmental factors, and genetic modification approaches in the literature. The intent of consolidating this information is to provide a starting resource for scientists considering how to optimize the parameters for cell differentiation and tissue culture procedures within the context of bone and cartilage tissue engineering.
在医疗保健领域,有一个非常引人关注且有巨大需求的领域,即骨骼和软骨的再生。多年来,许多组织工程策略已经为实现临床相关治疗方法做出了重大贡献。然而,细胞和组织培养方案存在许多差异,使得不同出版物之间的实验结果难以进行比较。本综述调查了文献中常见的细胞来源、可溶性因子、培养基配方、环境因素和遗传修饰方法。整合这些信息的目的是为科学家提供一个起点资源,帮助他们在骨骼和软骨组织工程的背景下,考虑如何优化细胞分化和组织培养过程的参数。