Whiteside T L, Heo D S, Takagi S, Herberman R B
Department of Pathology, University of Pittsburgh, PA.
Transplant Proc. 1988 Apr;20(2):347-50.
TIL were isolated from human solid tumors and cultured in rIL 2. Long-term TIL lines from primary tumors showed a shorter lag period, better expansion, and higher anti-tumor activity in vitro than those from metastatic tumors. The anti-tumor effector cells in TIL and A-PBL cultures had CD3+ Leu 19+ and CD3- Leu19+ phenotypes and morphology consistent with LGL/LAK cells. These cells mediated lysis of both autologous and allogeneic fresh tumor cell targets, while CD3+ Leu19- cells had little or no antitumor cytotoxicity.
肿瘤浸润淋巴细胞（TIL）从人实体瘤中分离出来，并在重组白细胞介素2（rIL 2）中培养。来自原发性肿瘤的长期TIL系在体外比来自转移性肿瘤的TIL系表现出更短的延迟期、更好的扩增能力和更高的抗肿瘤活性。TIL和活化外周血淋巴细胞（A-PBL）培养物中的抗肿瘤效应细胞具有CD3 + Leu 19 +和CD3 - Leu19 +表型，其形态与大颗粒淋巴细胞/淋巴因子激活的杀伤细胞（LGL/LAK细胞）一致。这些细胞介导对自体和异体新鲜肿瘤细胞靶标的裂解，而CD3 + Leu19 -细胞几乎没有或没有抗肿瘤细胞毒性。
