Kang Hyesook, Park Gyunam, Kim Hyeran, Chang Kyungsoo
Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan , Busan 609-757 , Korea.
JMM Case Rep. 2016 Feb 12;3(1):e005016. doi: 10.1099/jmmcr.0.005016. eCollection 2016 Feb.
(formerly known as ) is the causative agent of sore throat and also causes skin and soft tissue infections in diabetes patients. is a Gram-positive, catalase-negative, β-haemolytic bacillus. poses a diagnostic challenge in the hospital laboratory because most coryneform bacilli are considered as normal flora or contaminants, and it is therefore difficult to differentiate from β-haemolytic streptococci by colony characteristics.
was isolated from a diabetic patient with foot ulcers and the isolate was identified by using a VITEK-2 system, CAMP inhibition test, reverse CAMP test and a 23S rRNA gene sequence analysis. The isolated inhibited haemolysis of in the CAMP test and enhanced haemolysis of in the reverse CAMP test. The diabetic patient was treated with teicoplanin and imipenem, and the ulcers healed within 2 weeks.
The present study suggests that a haemolytic differential method using the CAMP inhibition and reverse CAMP tests can be useful for differentiating from β-haemolytic streptococci.
（曾用名 ）是咽痛的病原体，也可导致糖尿病患者发生皮肤和软组织感染。 是一种革兰氏阳性、过氧化氢酶阴性、β溶血杆菌。 在医院实验室中构成诊断挑战，因为大多数棒状杆菌被视为正常菌群或污染物，因此很难通过菌落特征与β溶血性链球菌区分开来。
从一名患有足部溃疡的糖尿病患者中分离出 ，并使用VITEK-2系统、CAMP抑制试验、反向CAMP试验和23S rRNA基因序列分析对分离株进行鉴定。分离出的 在CAMP试验中抑制 的溶血，并在反向CAMP试验中增强 的溶血。该糖尿病患者接受替考拉宁和亚胺培南治疗，溃疡在2周内愈合。
本研究表明，使用CAMP抑制试验和反向CAMP试验的溶血鉴别方法可有助于将 与β溶血性链球菌区分开来。
