2Department of Microbiology & Immunology, University of Melbourne, at the Peter Doherty Institute for Infection & Immunity, Melbourne, Australia.
1Doherty Applied Microbial Genomics, Department of Microbiology & Immunology, University of Melbourne, at the Peter Doherty Institute for Infection & Immunity, Melbourne, Australia.
Microb Genom. 2016 Aug 25;2(8):e000076. doi: 10.1099/mgen.0.000076. eCollection 2016 Aug.
Whole-genome sequencing (WGS) provides the highest resolution analysis for comparison of bacterial isolates in public health microbiology. However, although increasingly being used routinely for some pathogens such as and , the use of WGS is still limited for other organisms, such as . Multi-antigen sequence typing (NG-MAST) is the most widely performed typing method for epidemiological surveillance of gonorrhoea. Here, we present a command-line software tool for performing NG-MAST on assembled genome data. rapidly and accurately determined the NG-MAST of 630 assembled genomes, facilitating comparisons between WGS and previously published gonorrhoea epidemiological studies. The source code and user documentation are available at https://github.com/MDU-PHL/ngmaster.
全基因组测序 (WGS) 为公共卫生微生物学中细菌分离物的比较提供了最高分辨率的分析。然而，尽管 WGS 越来越多地用于某些病原体，如 和 ，但对于其他生物体，如 ，其使用仍然有限。多抗原序列分型 (NG-MAST) 是淋病流行病学监测中最广泛应用的分型方法。在这里，我们介绍了一种用于对组装基因组数据执行 NG-MAST 的命令行软件工具。该工具能够快速准确地确定 630 个组装基因组的 NG-MAST，促进了 WGS 与先前发表的淋病流行病学研究之间的比较。源代码和用户文档可在 https://github.com/MDU-PHL/ngmaster 上获得。
