增强辐射对HeLa癌细胞系的细胞毒性作用。
potentiates cytotoxic effects of radiation in HeLa cancer cell line.
作者信息
Hosseini Azar, Saeidi Javadi Shima, Fani-Pakdel Azar, Mousavi Seyed Hadi
机构信息
Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
Avicenna J Phytomed. 2017 Mar-Apr;7(2):137-144.
OBJECTIVE
Cervical cancer is the second most common cause of death from cancer in women throughout the world. The aim of this study was to evaluate the cytotoxic activity of () extract associated with radiotherapy in cervical cancer cells (HeLa cell line).
MATERIALS AND METHODS
Different concentration of the extract (25-500µg/ml) was tested in HeLa cell lines. Cell cytotoxicity of the extract and the effects of the extract on radiation (2Gy/min)-induced damages were assessed by MTT assay. Apoptosis was assessed using flow cytometric analysis.
RESULT
decreased cell viability in HeLa cell line in a concentration and time-dependent manner. When compared to the control, induced a sub-G1 peak in the flow cytometry histogram of treated cells, indicating that apoptotic cell death is involved in -induced toxicity. It was also shown that sensitizes cells to radiation-induced toxicity.
CONCLUSION
Our result showed the extract increased the radiation effect. This observation may be related to the presence of active compounds such as phthalides and ferulic acid.
目的
宫颈癌是全球女性癌症死亡的第二大常见原因。本研究的目的是评估（）提取物与放疗联合对宫颈癌细胞（HeLa细胞系）的细胞毒性作用。
材料与方法
在HeLa细胞系中测试不同浓度的提取物（25 - 500μg/ml）。通过MTT法评估提取物的细胞毒性以及提取物对辐射（2Gy/分钟）诱导损伤的影响。使用流式细胞术分析评估细胞凋亡。
结果
HeLa细胞系中的细胞活力以浓度和时间依赖性方式降低。与对照组相比，提取物在处理细胞的流式细胞术直方图中诱导出现亚G1峰，表明凋亡性细胞死亡参与了提取物诱导的毒性作用。还表明提取物使细胞对辐射诱导的毒性敏感。
结论
我们的结果表明该提取物增强了辐射效果。这一观察结果可能与诸如苯酞和阿魏酸等活性化合物的存在有关。
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