雄性Wistar大鼠肾结石形成及汤剂的抗氧化作用

Kidney stone formation and antioxidant effects of decoction in male Wistar rats.

作者信息

Golshan Alireza, Hayatdavoudi Parichehr, Hadjzadeh Mousa Al-Reza, Khajavi Rad Abolfazl, Mohamadian Roshan Nema, Abbasnezhad Abbasali, Mousavi Seyed Mojtaba, Pakdel Roghayeh, Zarei Batool, Aghaee Azita

机构信息

School of Medicine, North Khorasan University of Medical sciences, Bojnurd, Iran.

Neurocognitive Research Center, Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

出版信息

Avicenna J Phytomed. 2017 Mar-Apr;7(2):180-190.

PMID:28348973

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5355823/

Abstract

OBJECTIVES

The antioxidant capacity impairs in kidney and urinary bladder of animals with stone disease. Herbal medicine can improve the antioxidant condition of renal tissue. () is a medicinal plant with antioxidative and diuretic properties and different preparations of this plant have shown promising effects in stone disease. Assessment of the whole plant decoction to prevent kidney stone disease as well as its antioxidant effects was the aim of this paper.

MATERIALS AND METHODS

Fifty male Wistar rats were randomly divided into 5 experimental groups (n=10). One group was left without treatment and four groups received ethylene glycol (1% v/v) in drinking water for 6 weeks. Three doses of aqueous decoction (12.5, 50 and 200 mg/kg BW) were added to the drinking water of groups 3-5. Finally, water intake, 24-hour urine volume, MDA, total thiol concentration and FRAP value were measured in the serum and kidney tissues. The CaOx depositions were evaluated by hematoxylin and eosin staining.

RESULTS

Compared to the ethylene glycol-treated group, 200 mg/kg , lowered stone incidents, decreased urine volume, increased FRAP/g Cr (43%) and thiol content (p<0.05) with no significant alteration of water intake, MDA decreased significantly compared to 12.5 (p<0.01). Kidney weight increased and body weight decreased in ethylene glycol-treated group compared to the control group (p<0.05).

CONCLUSION

A minimum dose of 200 mg/kg reduced stone formation and simultaneously increased total antioxidant power of serum and preserved MDA content and water.

摘要

目的

结石病动物的肾脏和膀胱抗氧化能力受损。草药可改善肾组织的抗氧化状况。（某植物名称）是一种具有抗氧化和利尿特性的药用植物，该植物的不同制剂在结石病治疗中已显示出有前景的效果。本文旨在评估该植物全草水煎剂对肾结石病的预防作用及其抗氧化效果。

材料与方法

将50只雄性Wistar大鼠随机分为5个实验组（每组n = 10）。一组不进行处理，另外四组饮用含1%（体积/体积）乙二醇的水6周。在第3 - 5组的饮用水中添加三种剂量的水煎剂（12.5、50和200毫克/千克体重）。最后，测定血清和肾组织中的饮水量、24小时尿量、丙二醛（MDA）、总巯基浓度和铁还原抗氧化能力（FRAP）值。通过苏木精和伊红染色评估草酸钙沉积情况。

结果

与乙二醇处理组相比，200毫克/千克（该植物水煎剂）降低了结石发生率，减少了尿量，使FRAP/肌酐（Cr）增加了43%，巯基含量增加（p < 0.05），饮水量无显著变化，与12.5毫克/千克组相比，MDA显著降低（p < 0.01）。与对照组相比，乙二醇处理组肾脏重量增加，体重下降（p < 0.05）。