Rai D K, Sharma R K, Rai P K, Watal G, Sharma B
University of Allahabad, Departments of Biochemistry, Allahabad, India.
Cell Mol Biol (Noisy-le-grand). 2011 Feb 12;57(1):135-42.
The present study was designed to investigate the ameliorating effect of aqueous extract of C. dactylon on carbofuran induced oxidative stress (OS) and alterations in the activity of acetylcholinesterase (AChE) in the brain of rats. Vitamin C was used as a positive control. Wistar rats were administered with single sub-acute oral dose (1.6 mgkg-1 b.wt.) of carbofuran for 24 h. The OS parameters such as lipid peroxidation (LPO) and the activities of antioxidant enzymes including super oxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST), and that of AChE were studied in brain. Carbofuran treatment significantly increased the activities of SOD and CAT by 75 and 60%, respectively. It also induced the level of LPO by 113%. In contrast, the activities of GST and AChE were recorded to be diminished by 25 and 33%, respectively. Pretreatment of the rats with aqueous extract of C. dactylon (oral; 500mgkg-1) restored SOD activity completely but CAT activity only partially (7%). Carbofuran induced LPO was moderated by 95% in the brain of C. dactylon treated rats. The observed changes in OS parameters in C. dactylon treated group were comparable to that observed in vitamin C (200 mg-kg-1 b. wt.) treated group. Surprisingly, C. dactylon treatment significantly recovered the activity of AChE to a similar level as observed in the brain of control group. In contrast vitamin C treatment did not cause significant change in the activity of AChE in carbofuran treated group. There were no noticeable changes in the aforementioned study parameters in the brain of rats receiving C. dactylon and vitamin C, only. The results suggest that the study is extremely important in the context of development of new anticholinestesterase and antioxidant antidotes against carbofuran from C. dactylon.
本研究旨在探讨牛筋草水提取物对大鼠脑中呋喃丹诱导的氧化应激（OS）及乙酰胆碱酯酶（AChE）活性改变的改善作用。维生素C用作阳性对照。给Wistar大鼠单次口服亚急性剂量（1.6mg/kg体重）的呋喃丹，持续24小时。研究了大鼠脑中的OS参数，如脂质过氧化（LPO）以及抗氧化酶包括超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽-S-转移酶（GST）的活性，以及AChE的活性。呋喃丹处理使SOD和CAT的活性分别显著增加了75%和60%。它还使LPO水平升高了113%。相比之下，GST和AChE的活性分别降低了25%和33%。用牛筋草水提取物（口服；500mg/kg）预处理大鼠可使SOD活性完全恢复，但CAT活性仅部分恢复（7%）。在牛筋草处理的大鼠脑中，呋喃丹诱导的LPO降低了95%。在牛筋草处理组中观察到的OS参数变化与在维生素C（200mg/kg体重）处理组中观察到的变化相当。令人惊讶的是，牛筋草处理使AChE的活性显著恢复到与对照组脑中观察到的相似水平。相比之下，维生素C处理在呋喃丹处理组中并未引起AChE活性的显著变化。仅接受牛筋草和维生素C的大鼠脑中上述研究参数没有明显变化。结果表明，该研究在开发针对呋喃丹的新型抗胆碱酯酶和抗氧化解毒剂方面具有极其重要的意义。
