Madden Christopher J, Santos da Conceicao Ellen Paula, Morrison Shaun F
Department of Neurological Surgery, Oregon Health & Science University , Portland, OR, USA.
Temperature (Austin). 2016 Nov 10;4(1):89-96. doi: 10.1080/23328940.2016.1257407. eCollection 2017.
In urethane/α-chloralose anesthetized rats, electrical stimulation of cervical vagal afferent fibers inhibited the increases in brown adipose tissue sympathetic nerve activity and brown adipose tissue thermogenesis evoked by cold exposure, by nanoinjection of the GABA receptor antagonist, bicuculline, in the dorsomedial hypothalamus, and by nanoinjection of N-methyl-D-aspartate in the rostral raphe pallidus. Vagus nerve stimulation-evoked inhibition of brown adipose tissue sympathetic nerve activity was prevented by blockade of ionotropic glutamate receptors in the termination site of vagal afferents in the nucleus of the solitary tract, and by nanoinjection of GABA receptor antagonists in the rostral raphe pallidus. In conclusion, the brown adipose tissue sympathoinhibitory effect of cervical afferent vagal nerve stimulation is mediated by glutamatergic activation of second-order sensory neurons in the nucleus of the solitary tract and by a GABAergic inhibition of brown adipose tissue sympathetic premotor neurons in the rostral raphe pallidus, but does not require GABAergic inhibition of the brown adipose tissue sympathoexcitatory neurons in the dorsomedial hypothalamus.
在氨基甲酸乙酯/α-氯醛糖麻醉的大鼠中，电刺激颈迷走神经传入纤维可抑制冷暴露引起的棕色脂肪组织交感神经活动增加和棕色脂肪组织产热，这种抑制作用可通过在背内侧下丘脑微量注射GABA受体拮抗剂荷包牡丹碱，以及在吻侧中缝苍白核微量注射N-甲基-D-天冬氨酸来实现。迷走神经刺激诱发的棕色脂肪组织交感神经活动抑制，可通过阻断孤束核中迷走神经传入终末部位的离子型谷氨酸受体，以及在吻侧中缝苍白核微量注射GABA受体拮抗剂来防止。总之，颈迷走神经传入神经刺激对棕色脂肪组织的交感抑制作用，是由孤束核中二级感觉神经元的谷氨酸能激活以及吻侧中缝苍白核中棕色脂肪组织交感运动前神经元的GABA能抑制介导的，但并不需要背内侧下丘脑棕色脂肪组织交感兴奋神经元的GABA能抑制。
