Butte N F, Wong W W, Patterson B W, Garza C, Klein P D
US Department of Agriculture, Children's Nutrition Research Center, Houston, TX 77030.
Am J Clin Nutr. 1988 May;47(5):815-21. doi: 10.1093/ajcn/47.5.815.
A comparison was made between the dose-to-the-mother deuterium-dilution method and the conventional test-weighing technique for determining human-milk intake in five exclusively breast-fed infants and in four breast-fed infants who received supplemental foods. After administration of 2H to the mothers human milk and infant urine were sampled over 14 d and analyzed for 2H:1H ratios by gas-isotope-ratio mass spectrometry. Infant total body water was determined by 18O dilution. The test-weighing procedure was conducted for 5 d consecutively. The intake of human milk (mean +/- SD) estimated by 2H dilution was 648 +/- 63 g/d and estimated by test-weighing was 636 +/- 84 g/d. The mean difference between the two methods was not significantly different from 0. The 2H-dilution and test-weighing techniques provide similar estimates of human-milk intake.
对五名纯母乳喂养婴儿和四名接受辅食的母乳喂养婴儿，采用母亲体内氘稀释法和传统称重技术来测定母乳摄入量，并进行了比较。给母亲服用2H后，在14天内采集母乳和婴儿尿液样本，并用气体同位素比值质谱法分析2H:1H比值。通过18O稀释法测定婴儿全身水含量。连续5天进行称重程序。通过2H稀释法估算的母乳摄入量（平均值±标准差）为648±63克/天，通过称重法估算的为636±84克/天。两种方法的平均差异与0无显著差异。2H稀释法和称重技术对母乳摄入量的估算结果相似。
