高通量RNA亚硫酸氢盐测序数据分析

Analysis of High-Throughput RNA Bisulfite Sequencing Data.

作者信息

Rieder Dietmar, Finotello Francesca

机构信息

Division of Bioinformatics, Biocenter, Medical University of Innsbruck, Innrain 80/IV, Innsbruck, 6020, Austria.

出版信息

Methods Mol Biol. 2017;1562:143-154. doi: 10.1007/978-1-4939-6807-7_10.

DOI:10.1007/978-1-4939-6807-7_10

PMID:28349459

Abstract

Methylation of the 5-cytosine (mC) is a common but not well-understood RNA modification, which can be detected by sequencing of bisulfite-treated transcripts (RNA-BSseq). In this Chapter, we discuss computational RNA-BSseq data analysis methods for transcriptome-wide identification and quantification of mC.

摘要

5-胞嘧啶（mC）的甲基化是一种常见但尚未被充分理解的RNA修饰，可通过亚硫酸氢盐处理的转录本测序（RNA-BSseq）进行检测。在本章中，我们将讨论用于全转录组范围内mC鉴定和定量的计算RNA-BSseq数据分析方法。