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通过mC RNA免疫沉淀结合深度测序在植物中进行全转录组范围的5-甲基胞嘧啶定位

Transcriptome-Wide Mapping 5-Methylcytosine by mC RNA Immunoprecipitation Followed by Deep Sequencing in Plant.

作者信息

Gu Xiaofeng, Liang Zhe

机构信息

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.

Department of Biological Sciences, National University of Singapore, Singapore, Singapore.

出版信息

Methods Mol Biol. 2019;1933:389-394. doi: 10.1007/978-1-4939-9045-0_24.

DOI:10.1007/978-1-4939-9045-0_24
PMID:30945199
Abstract

Transcriptome-wide mapping RNA modification is crucial to understand the distribution and function of RNA modifications. Here, we describe a protocol to transcriptome-wide mapping 5-methylcytosine (mC) in plant, by a RNA immunoprecipitation followed by deep sequencing (mC-RIP-seq) approach. The procedure includes RNA extraction, fragmentation, RNA immunoprecipitation, and library construction.

摘要

全转录组范围的RNA修饰图谱绘制对于理解RNA修饰的分布和功能至关重要。在此，我们描述了一种通过RNA免疫沉淀后深度测序（mC-RIP-seq）方法在植物中进行全转录组范围5-甲基胞嘧啶（mC）图谱绘制的方案。该程序包括RNA提取、片段化、RNA免疫沉淀和文库构建。

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