Lawson Charlotte, Rose Marlene, Wolf Sabine
Comparative Biomedical Sciences, Royal Veterinary College, Royal College Street, London, NW1 0TU, UK.
Harefield Hospital, Imperial College, London, UB9 6JH, UK.
Methods Mol Biol. 2017;1591:85-100. doi: 10.1007/978-1-4939-6931-9_7.
Vascular endothelial cells (ECs) line the luminal side of all blood vessels and act as a selective barrier between blood and tissue. ECs are constantly exposed to biochemical and biomechanical stimuli from the blood and underlying tissue. Fluid shear stress acts in parallel to the vessel wall, resulting from friction of blood against EC. Despite the importance of flow on normal EC function, much of the information regarding EC function and dysfunction has been derived from cells harvested, grown, and studied in static culture.In order to study the effects of shear stress on EC function a number of in vitro models have been developed. This manuscript provides methodology for use of a system which enables recirculation of leukocytes and cell culture medium over the endothelium for a period of several minutes to days and enables investigation of the effects of prolonged leukocyte coculture on both the endothelial and leukocyte populations.
血管内皮细胞（ECs）排列在所有血管的管腔侧，作为血液与组织之间的选择性屏障。内皮细胞不断受到来自血液和下层组织的生化和生物力学刺激。流体剪切应力与血管壁平行作用，是血液与内皮细胞摩擦产生的。尽管血流对正常内皮细胞功能很重要，但许多关于内皮细胞功能和功能障碍的信息都来自于在静态培养中收获、培养和研究的细胞。为了研究剪切应力对内皮细胞功能的影响，已经开发了一些体外模型。本手稿提供了一种系统的使用方法，该系统能够使白细胞和细胞培养基在内皮上再循环数分钟至数天，并能够研究长时间白细胞共培养对内皮细胞和白细胞群体的影响。
